Recombinant
RabMAb

Anti-67kDa Laminin Receptor antibody [EPR8469] (Biotin) (ab198348)

Overview

  • Product name
    Anti-67kDa Laminin Receptor antibody [EPR8469] (Biotin)
    See all 67kDa Laminin Receptor primary antibodies
  • Description
    Rabbit monoclonal [EPR8469] to 67kDa Laminin Receptor (Biotin)
  • Host species
    Rabbit
  • Conjugation
    Biotin
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human 67kDa Laminin Receptor aa 250 to the C-terminus (C terminal). The exact sequence is proprietary.

  • Positive control
    • IHC-P: human colon adenocarcinoma tissue sections
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab198348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis. Acts as a PPP1R16B-dependent substrate of PPP1CA. Also acts as a receptor for several other ligands, including the pathogenic prion protein, viruses, and bacteria.
  • Sequence similarities
    Belongs to the ribosomal protein S2P family.
  • Post-translational
    modifications
    Acylated. Acylation may be a prerequisite for conversion of the monomeric 37 kDa laminin receptor precursor (37LRP) to the mature dimeric 67 kDa laminin receptor (67LR), and may provide a mechanism for membrane association.
    Cleaved by stromelysin-3 (ST3) at the cell surface. Cleavage by stromelysin-3 may be a mechanism to alter cell-extracellular matrix interactions.
  • Cellular localization
    Cell membrane. Cytoplasm. Nucleus. 67LR is found at the surface of the plasma membrane, with its C-terminal laminin-binding domain accessible to extracellular ligands. 37LRP is found at the cell surface, in the cytoplasm and in the nucleus (By similarity). Co-localizes with PPP1R16B in the cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 34/67 kDa laminin receptor antibody
    • 37 kDa laminin receptor precursor antibody
    • 37/67 kDa laminin receptor antibody
    • 37LRP antibody
    • 40S ribosomal protein SA antibody
    • 67 kDa laminin receptor antibody
    • 67LR antibody
    • Colon carcinoma laminin binding protein antibody
    • Colon carcinoma laminin-binding protein antibody
    • LAMBR antibody
    • Laminin receptor 1 antibody
    • Laminin-binding protein precursor p40 antibody
    • LAMR 1 antibody
    • LamR antibody
    • LAMR1 antibody
    • LBP antibody
    • LBP/p40 antibody
    • LRP antibody
    • LRP/LR antibody
    • Multidrug resistance associated protein MGr1 Ag antibody
    • Multidrug resistance associated protein MGr1Ag antibody
    • Multidrug resistance-associated protein MGr1-Ag antibody
    • NEM/1CHD4 antibody
    • p40 antibody
    • Ribosomal Protein SA antibody
    • rpsA antibody
    • RSSA_HUMAN antibody
    • SA antibody
    see all

Images

  • IHC image of 67kDa Laminin Receptor staining in a section of formalin-fixed paraffin-embedded human colon adenocarcinoma*, performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab198348, 1/100 dilution, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

References

ab198348 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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