Overview

  • Product name
    Anti-67kDa Laminin Receptor antibody [EPR8469]
    See all 67kDa Laminin Receptor primary antibodies
  • Description
    Rabbit monoclonal [EPR8469] to 67kDa Laminin Receptor
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human 67kDa Laminin Receptor aa 250-350 (C terminal). The exact sequence is proprietary.

  • Positive control
    • K562, HeLa, HepG2, C6, RAW 264.7, PC-12 and NIH/3T3 cell lysates; Human breast and kidney tissues
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133645 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 33 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
Flow Cyt 1/20 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis. Acts as a PPP1R16B-dependent substrate of PPP1CA. Also acts as a receptor for several other ligands, including the pathogenic prion protein, viruses, and bacteria.
  • Sequence similarities
    Belongs to the ribosomal protein S2P family.
  • Post-translational
    modifications
    Acylated. Acylation may be a prerequisite for conversion of the monomeric 37 kDa laminin receptor precursor (37LRP) to the mature dimeric 67 kDa laminin receptor (67LR), and may provide a mechanism for membrane association.
    Cleaved by stromelysin-3 (ST3) at the cell surface. Cleavage by stromelysin-3 may be a mechanism to alter cell-extracellular matrix interactions.
  • Cellular localization
    Cell membrane. Cytoplasm. Nucleus. 67LR is found at the surface of the plasma membrane, with its C-terminal laminin-binding domain accessible to extracellular ligands. 37LRP is found at the cell surface, in the cytoplasm and in the nucleus (By similarity). Co-localizes with PPP1R16B in the cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 34/67 kDa laminin receptor antibody
    • 37 kDa laminin receptor precursor antibody
    • 37/67 kDa laminin receptor antibody
    • 37LRP antibody
    • 40S ribosomal protein SA antibody
    • 67 kDa laminin receptor antibody
    • 67LR antibody
    • Colon carcinoma laminin binding protein antibody
    • Colon carcinoma laminin-binding protein antibody
    • LAMBR antibody
    • Laminin receptor 1 antibody
    • Laminin-binding protein precursor p40 antibody
    • LAMR 1 antibody
    • LamR antibody
    • LAMR1 antibody
    • LBP antibody
    • LBP/p40 antibody
    • LRP antibody
    • LRP/LR antibody
    • Multidrug resistance associated protein MGr1 Ag antibody
    • Multidrug resistance associated protein MGr1Ag antibody
    • Multidrug resistance-associated protein MGr1-Ag antibody
    • NEM/1CHD4 antibody
    • p40 antibody
    • Ribosomal Protein SA antibody
    • rpsA antibody
    • RSSA_HUMAN antibody
    • SA antibody
    see all

Images

  • All lanes : Anti-67kDa Laminin Receptor antibody [EPR8469] (ab133645) at 1/10000 dilution

    Lane 1 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 3 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 33 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

  • ab133645 staining Prohibitin in mouse kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

    Negative control 1: PBS in place of primary antibody.

  • ab133645 staining 67kDa Laminin Receptor in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/250. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. ab195889 was used as a counterstain for primary antibody ab133645 at 1/2000. DAPI was used as a nuclear counterstain and PBS as a negative control.

  • ab133645 staining 67kDa Laminin Receptor in the human cell line MCF7 (Human breast adenocarcinoma cell line) by flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/20. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: Rabbit monoclonal IgG (Black)

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

  • ab133645 immunoprecipitating 67kDa Laminin Receptor. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/20 and VeriBlot for IP secondary antibody (HRP) (ab131366) at a dilution of 1/1000.

    Lane 1: K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate (10ug)
    Lane 2: K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab133645 in K562(Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate

  • ab133645 staining 67kDa Laminin Receptor in rat kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

    Negative control 1: PBS in place of primary antibody.

  • ab133645 staining 67kDa Laminin Receptor in mouse kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

    Negative control 1: PBS in place of primary antibody.

  • ab133645 staining 67kDa Laminin Receptor in human gastric carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

    Negative control 1: PBS in place of primary antibody.

  • All lanes : Anti-67kDa Laminin Receptor antibody [EPR8469] (ab133645) at 1/2000 dilution

    Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 4 : C6 (Rat glial tumor cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 33 kDa



    Diluting and blocking buffer: 5% NFDM/TBST

  • Overlay histogram showing MCF7 cells stained with ab133645 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133645, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • All lanes : Anti-67kDa Laminin Receptor antibody [EPR8469] (ab133645) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : C6 cell lysate
    Lane 5 : RAW 264.7 cell lysate
    Lane 6 : PC-12 cell lysate
    Lane 7 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 33 kDa

  • Immunohistochemical analysis of paraffin-embedded Human breast tissue labelling 67kDa Laminin Receptor with ab133645 at 1/100 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labellig 67kDa Laminin Receptor with ab133645 at 1/100 dilution.
  • All lanes : Anti-67kDa Laminin Receptor antibody [EPR8469] (ab133645) at 1/1000 dilution

    All lanes : Mouse intestinal mucosa whole tissue lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/2500 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 33 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute

    See Abreview

References

This product has been referenced in:
  • Gao S  et al. PEDF mediates pathological neovascularization by regulating macrophage recruitment and polarization in the mouse model of oxygen-induced retinopathy. Sci Rep 7:42846 (2017). Read more (PubMed: 28211523) »
  • Guo J  et al. Promoting potential of adipose derived stem cells on peripheral nerve regeneration. Mol Med Rep 16:7297-7304 (2017). Read more (PubMed: 28944869) »
See all 3 Publications for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (12)
Sample
Mouse Tissue lysate - whole (intestinal mucosa)
Specification
intestinal mucosa
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Mar 27 2014

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