Anti-67kDa Laminin Receptor antibody (ab110499)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-67kDa Laminin Receptor antibody
See all 67kDa Laminin Receptor primary antibodies -
Description
Rabbit polyclonal to 67kDa Laminin Receptor -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Chicken, Cow, Pig, Zebrafish, Zebra finch
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Immunogen
Synthetic peptide corresponding to Human 67kDa Laminin Receptor aa 150-250 conjugated to keyhole limpet haemocyanin.
(Peptide available asab111325) -
Positive control
- Recombinant Human 67kDa Laminin Receptor protein (ab114294) can be used as a positive control in WB. This antibody gave a positive signal in the following whole cell lysates: Caco2; HCT116; LOVO; A549; HeLa.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab110499 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 33 kDa). |
Target
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Function
Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis. Acts as a PPP1R16B-dependent substrate of PPP1CA. Also acts as a receptor for several other ligands, including the pathogenic prion protein, viruses, and bacteria. -
Sequence similarities
Belongs to the ribosomal protein S2P family. -
Post-translational
modificationsAcylated. Acylation may be a prerequisite for conversion of the monomeric 37 kDa laminin receptor precursor (37LRP) to the mature dimeric 67 kDa laminin receptor (67LR), and may provide a mechanism for membrane association.
Cleaved by stromelysin-3 (ST3) at the cell surface. Cleavage by stromelysin-3 may be a mechanism to alter cell-extracellular matrix interactions. -
Cellular localization
Cell membrane. Cytoplasm. Nucleus. 67LR is found at the surface of the plasma membrane, with its C-terminal laminin-binding domain accessible to extracellular ligands. 37LRP is found at the cell surface, in the cytoplasm and in the nucleus (By similarity). Co-localizes with PPP1R16B in the cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 281898 Cow
- Entrez Gene: 3921 Human
- Entrez Gene: 100505031 Mouse
- Entrez Gene: 16785 Mouse
- Entrez Gene: 641351 Pig
- Entrez Gene: 29236 Rat
- Entrez Gene: 394027 Zebrafish
- Omim: 150370 Human
see all -
Alternative names
- 34/67 kDa laminin receptor antibody
- 37 kDa laminin receptor precursor antibody
- 37/67 kDa laminin receptor antibody
see all
Images
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Western blot - Anti-67kDa Laminin Receptor antibody (ab110499)All lanes : Anti-67kDa Laminin Receptor antibody (ab110499) at 1 µg/ml
Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 2 : HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate
Lane 3 : LOVO (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lane 4 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Additional bands at: 19 kDa, 94 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
Protocols
Datasheets and documents
References
ab110499 has not yet been referenced specifically in any publications.
