Key features and details
- Rabbit polyclonal to 67kDa Laminin Receptor
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-67kDa Laminin Receptor antibody
See all 67kDa Laminin Receptor primary antibodies
DescriptionRabbit polyclonal to 67kDa Laminin Receptor
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Pig, Monkey
Recombinant fragment, corresponding to a region within amino acids 1-294 of Human 67kDa Laminin Receptor (Uniprot ID: P08865).
- 293T, A431, H1299, HeLa, HepG2, MOLT4, Raji and Mouse brain whole cell lysates; HeLa cells; Human CA922 xenograft tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab137388 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/1000.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer (pH8.0)|
|WB||1/500 - 1/3000. Predicted molecular weight: 33 kDa.|
FunctionRequired for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis. Acts as a PPP1R16B-dependent substrate of PPP1CA. Also acts as a receptor for several other ligands, including the pathogenic prion protein, viruses, and bacteria.
Sequence similaritiesBelongs to the ribosomal protein S2P family.
modificationsAcylated. Acylation may be a prerequisite for conversion of the monomeric 37 kDa laminin receptor precursor (37LRP) to the mature dimeric 67 kDa laminin receptor (67LR), and may provide a mechanism for membrane association.
Cleaved by stromelysin-3 (ST3) at the cell surface. Cleavage by stromelysin-3 may be a mechanism to alter cell-extracellular matrix interactions.
Cellular localizationCell membrane. Cytoplasm. Nucleus. 67LR is found at the surface of the plasma membrane, with its C-terminal laminin-binding domain accessible to extracellular ligands. 37LRP is found at the cell surface, in the cytoplasm and in the nucleus (By similarity). Co-localizes with PPP1R16B in the cell membrane.
- Information by UniProt
- 34/67 kDa laminin receptor antibody
- 37 kDa laminin receptor precursor antibody
- 37/67 kDa laminin receptor antibody
Anti-67kDa Laminin Receptor antibody (ab137388) at 1/1000 dilution + HepG2 whole cell lysate
at 30 µg
Predicted band size: 33 kDa
12% SDS PAGE
Anti-67kDa Laminin Receptor antibody (ab137388) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 33 kDa
12% SDS PAGE
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labelling 67kDa Laminin Receptor with ab137388 at 1/200 dilution. Lower image is merged with DNA probe.
Immunohistochemical analysis of paraffin-embedded Human CA922 xenograft tissue, labelling 67kDa Laminin Receptor with ab137388 at 1/500 dilution.
ab137388 has been referenced in 3 publications.
- Garita-Hernandez M et al. AAV-Mediated Gene Delivery to 3D Retinal Organoids Derived from Human Induced Pluripotent Stem Cells. Int J Mol Sci 21:N/A (2020). PubMed: 32028585
- Ashok A et al. Prion protein modulates endothelial to mesenchyme-like transition in trabecular meshwork cells: Implications for primary open angle glaucoma. Sci Rep 9:13090 (2019). PubMed: 31511544
- Khajuria RK et al. Ribosome Levels Selectively Regulate Translation and Lineage Commitment in Human Hematopoiesis. Cell 173:90-103.e19 (2018). PubMed: 29551269