For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    67kda-laminin-receptor-antibody-epr8468-ab133775.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cardiovascular Angiogenesis Adhesion / ECM Extracellular Matrix
Share by email
RecombinantRabMAb

Recombinant Anti-67kDa Laminin Receptor antibody [EPR8468] (ab133775)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (2)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-67kDa Laminin Receptor antibody [EPR8468] (ab133775)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-67kDa Laminin Receptor antibody [EPR8468] (ab133775)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-67kDa Laminin Receptor antibody [EPR8468] (ab133775)
  • OI-RD Scanning - Anti-67kDa Laminin Receptor antibody [EPR8468] (ab133775)
  • Anti-67kDa Laminin Receptor antibody [EPR8468] (ab133775)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8468] to 67kDa Laminin Receptor
  • Suitable for: WB, IHC-P
  • Reacts with: Human

You may also be interested in

Protein
Product image
Recombinant Human 67kDa Laminin Receptor protein (ab114294)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

View more associated products

Overview

  • Product name

    Anti-67kDa Laminin Receptor antibody [EPR8468]
    See all 67kDa Laminin Receptor primary antibodies
  • Description

    Rabbit monoclonal [EPR8468] to 67kDa Laminin Receptor
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    A synthetic peptide corresponding to residues in Human 67kDa Laminin Receptor (UniProt ID: P08865)

  • Positive control

    • WB: Lysates of K562, HeLa, HepG2, C6, RAW 264.7, PC 12, NIH/3T3 IHC-P: Human brain tissue, Human kidney tissue
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 6.70 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8468
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Adhesion / ECM
    • Extracellular Matrix
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Prions
    • Neuroscience
    • Cell Adhesion Proteins
    • Cytoskeletal Proteins
    • Intermediate Filaments
    • Signal Transduction
    • Cytoskeleton / ECM
    • Basal Lamina
    • Neuroscience
    • Cell Adhesion Proteins
    • ECM Proteins

Associated products

  • Alternative Versions

    • Anti-67kDa Laminin Receptor antibody [EPR8468] - BSA and Azide free (ab248653)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • NIH/3T3 cytoplasmic extract lysate (ab14873)
    • HeLa membrane extract lysate (ab29547)
    • K-562 whole cell lysate (ab7911)
  • Recombinant Protein

    • Recombinant Human 67kDa Laminin Receptor protein (ab114294)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
All applications
Mouse
Rat
Application Abreviews Notes
WB (1)
1/1000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 33 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 33 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC or IP.
  • Target

    • Function

      Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis. Acts as a PPP1R16B-dependent substrate of PPP1CA. Also acts as a receptor for several other ligands, including the pathogenic prion protein, viruses, and bacteria.
    • Sequence similarities

      Belongs to the ribosomal protein S2P family.
    • Post-translational
      modifications

      Acylated. Acylation may be a prerequisite for conversion of the monomeric 37 kDa laminin receptor precursor (37LRP) to the mature dimeric 67 kDa laminin receptor (67LR), and may provide a mechanism for membrane association.
      Cleaved by stromelysin-3 (ST3) at the cell surface. Cleavage by stromelysin-3 may be a mechanism to alter cell-extracellular matrix interactions.
    • Cellular localization

      Cell membrane. Cytoplasm. Nucleus. 67LR is found at the surface of the plasma membrane, with its C-terminal laminin-binding domain accessible to extracellular ligands. 37LRP is found at the cell surface, in the cytoplasm and in the nucleus (By similarity). Co-localizes with PPP1R16B in the cell membrane.
    • Target information above from: UniProt accession P08865 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 3921 Human
      • Entrez Gene: 100505031 Mouse
      • Entrez Gene: 16785 Mouse
      • Entrez Gene: 29236 Rat
      • Omim: 150370 Human
      • SwissProt: P08865 Human
      • SwissProt: P14206 Mouse
      • SwissProt: P38983 Rat
      • Unigene: 449909 Human
      • Unigene: 311972 Mouse
      • Unigene: 391708 Mouse
      • Unigene: 4071 Mouse
      • Unigene: 121972 Rat
      • Unigene: 161973 Rat
      • Unigene: 999 Rat
      see all
    • Alternative names

      • 34/67 kDa laminin receptor antibody
      • 37 kDa laminin receptor precursor antibody
      • 37/67 kDa laminin receptor antibody
      • 37LRP antibody
      • 40S ribosomal protein SA antibody
      • 67 kDa laminin receptor antibody
      • 67LR antibody
      • Colon carcinoma laminin binding protein antibody
      • Colon carcinoma laminin-binding protein antibody
      • LAMBR antibody
      • Laminin receptor 1 antibody
      • Laminin-binding protein precursor p40 antibody
      • LAMR 1 antibody
      • LamR antibody
      • LAMR1 antibody
      • LBP antibody
      • LBP/p40 antibody
      • LRP antibody
      • LRP/LR antibody
      • Multidrug resistance associated protein MGr1 Ag antibody
      • Multidrug resistance associated protein MGr1Ag antibody
      • Multidrug resistance-associated protein MGr1-Ag antibody
      • NEM/1CHD4 antibody
      • p40 antibody
      • Ribosomal Protein SA antibody
      • rpsA antibody
      • RSSA_HUMAN antibody
      • SA antibody
      see all

    Images

    • Western blot - Anti-67kDa Laminin Receptor antibody [EPR8468] (ab133775)
      Western blot - Anti-67kDa Laminin Receptor antibody [EPR8468] (ab133775)
      All lanes : Anti-67kDa Laminin Receptor antibody [EPR8468] (ab133775) at 1/2000 dilution

      Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
      Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
      Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line)
      Lane 4 : SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 33 kDa
      Observed band size: 43 kDa why is the actual band size different from the predicted?



      Blocking/Diluting buffer and concentration: 5% NFDM/TBST

      Exposure time: 60 seconds.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-67kDa Laminin Receptor antibody [EPR8468] (ab133775)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-67kDa Laminin Receptor antibody [EPR8468] (ab133775)

      ab133775, at 1/250, staining 67kDa Laminin Receptor in paraffin-embedded Human brain tissue using immunohistochemical analysis.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-67kDa Laminin Receptor antibody [EPR8468] (ab133775)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-67kDa Laminin Receptor antibody [EPR8468] (ab133775)

      ab133775, at 1/250, staining 67kDa Laminin Receptor in paraffin-embedded Human kidney tissue using immunohistochemical analysis.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • OI-RD Scanning - Anti-67kDa Laminin Receptor antibody [EPR8468] (ab133775)
      OI-RD Scanning - Anti-67kDa Laminin Receptor antibody [EPR8468] (ab133775)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-67kDa Laminin Receptor antibody [EPR8468] (ab133775)
      Anti-67kDa Laminin Receptor antibody [EPR8468] (ab133775)

    Protocols

    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab133775? Please let us know so that we can cite the reference in this datasheet.

    ab133775 has been referenced in 2 publications.

    • Park H  et al. Dysfunction of 67-kDa Laminin Receptor Disrupts BBB Integrity via Impaired Dystrophin/AQP4 Complex and p38 MAPK/VEGF Activation Following Status Epilepticus. Front Cell Neurosci 13:236 (2019). PubMed: 31178701
    • Park H & Kang TC The Regional Specific Alterations in BBB Permeability are Relevant to the Differential Responses of 67-kDa LR Expression in Endothelial Cells and Astrocytes Following Status Epilepticus. Int J Mol Sci 20:N/A (2019). PubMed: 31795399

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-67kDa Laminin Receptor antibody [EPR8468]

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (BRAIN)
    Gel Running Conditions
    Reduced Non-Denaturing (Native) (4-20% gradient)
    Loading amount
    40 µg
    Specification
    BRAIN
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 27 2019

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.