Recombinant
RabMAb

Recombinant Anti-67kDa Laminin Receptor antibody [EPR8468] (ab133775)

Reviews (1) Submit a question References (1)

Overview

  • Product name

    Anti-67kDa Laminin Receptor antibody [EPR8468]
    See all 67kDa Laminin Receptor primary antibodies

  • Description

    Rabbit monoclonal [EPR8468] to 67kDa Laminin Receptor

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    A synthetic peptide corresponding to residues in Human 67kDa Laminin Receptor (UniProt ID: P08865)

  • Positive control

    • WB: Lysates of K562, HeLa, HepG2, C6, RAW 264.7, PC 12, NIH/3T3 IHC-P: Human brain tissue, Human kidney tissue

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab133775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 33 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC or IP.

    • Target

    • Function

      Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis. Acts as a PPP1R16B-dependent substrate of PPP1CA. Also acts as a receptor for several other ligands, including the pathogenic prion protein, viruses, and bacteria.

    • Sequence similarities

      Belongs to the ribosomal protein S2P family.

    • Post-translational
      modifications

      Acylated. Acylation may be a prerequisite for conversion of the monomeric 37 kDa laminin receptor precursor (37LRP) to the mature dimeric 67 kDa laminin receptor (67LR), and may provide a mechanism for membrane association.
      Cleaved by stromelysin-3 (ST3) at the cell surface. Cleavage by stromelysin-3 may be a mechanism to alter cell-extracellular matrix interactions.

    • Cellular localization

      Cell membrane. Cytoplasm. Nucleus. 67LR is found at the surface of the plasma membrane, with its C-terminal laminin-binding domain accessible to extracellular ligands. 37LRP is found at the cell surface, in the cytoplasm and in the nucleus (By similarity). Co-localizes with PPP1R16B in the cell membrane.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • 34/67 kDa laminin receptor antibody
      • 37 kDa laminin receptor precursor antibody
      • 37/67 kDa laminin receptor antibody
      • 37LRP antibody
      • 40S ribosomal protein SA antibody
      • 67 kDa laminin receptor antibody
      • 67LR antibody
      • Colon carcinoma laminin binding protein antibody
      • Colon carcinoma laminin-binding protein antibody
      • LAMBR antibody
      • Laminin receptor 1 antibody
      • Laminin-binding protein precursor p40 antibody
      • LAMR 1 antibody
      • LamR antibody
      • LAMR1 antibody
      • LBP antibody
      • LBP/p40 antibody
      • LRP antibody
      • LRP/LR antibody
      • Multidrug resistance associated protein MGr1 Ag antibody
      • Multidrug resistance associated protein MGr1Ag antibody
      • Multidrug resistance-associated protein MGr1-Ag antibody
      • NEM/1CHD4 antibody
      • p40 antibody
      • Ribosomal Protein SA antibody
      • rpsA antibody
      • RSSA_HUMAN antibody
      • SA antibody
      see all

    Images

    • Western blot - Anti-67kDa Laminin Receptor antibody [EPR8468] (ab133775)
      Western blot - Anti-67kDa Laminin Receptor antibody [EPR8468] (ab133775)
      All lanes : Anti-67kDa Laminin Receptor antibody [EPR8468] (ab133775) at 1/2000 dilution

      Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
      Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
      Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line)
      Lane 4 : SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 33 kDa
      Observed band size: 43 kDa
      why is the actual band size different from the predicted?



      Blocking/Diluting buffer and concentration: 5% NFDM/TBST

      Exposure time: 60 seconds.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-67kDa Laminin Receptor antibody [EPR8468] (ab133775)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-67kDa Laminin Receptor antibody [EPR8468] (ab133775)

      ab133775, at 1/250, staining 67kDa Laminin Receptor in paraffin-embedded Human brain tissue using immunohistochemical analysis.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-67kDa Laminin Receptor antibody [EPR8468] (ab133775)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-67kDa Laminin Receptor antibody [EPR8468] (ab133775)

      ab133775, at 1/250, staining 67kDa Laminin Receptor in paraffin-embedded Human kidney tissue using immunohistochemical analysis.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Other - Anti-67kDa Laminin Receptor antibody [EPR8468] (ab133775)
      Other - Anti-67kDa Laminin Receptor antibody [EPR8468] (ab133775)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Park H  et al. Dysfunction of 67-kDa Laminin Receptor Disrupts BBB Integrity via Impaired Dystrophin/AQP4 Complex and p38 MAPK/VEGF Activation Following Status Epilepticus. Front Cell Neurosci 13:236 (2019). Read more (PubMed: 31178701) »
    See 1 Publication for this product

    Publishing research using ab133775? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-67kDa Laminin Receptor antibody [EPR8468]

     Good
    Abreviews
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (BRAIN)
    Gel Running Conditions
    Reduced Non-Denaturing (Native) (4-20% gradient)
    Loading amount
    40 µg
    Specification
    BRAIN
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 27 2019

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com