Overview

  • Product name
    Anti-68kDa Neurofilament / NF-L antibody
    See all 68kDa Neurofilament / NF-L primary antibodies
  • Description
    Rabbit polyclonal to 68kDa Neurofilament / NF-L
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human 68kDa Neurofilament/ NF-L (internal sequence).

  • Positive control
    • Human lung carcinoma tissue
  • General notes

    Previously labelled as 68kDa Neurofilament. 

Properties

Applications

Our Abpromise guarantee covers the use of ab135884 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
  • Involvement in disease
    Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 1F (CMT1F) [MIM:607734]. CMT1F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. CMT1F is characterized by onset in infancy or childhood (range 1 to 13 years).
    Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 2E (CMT2E) [MIM:607684]. CMT2E is an autosomal dominant form of Charcot-Marie-Tooth disease type 2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy.
  • Sequence similarities
    Belongs to the intermediate filament family.
  • Domain
    The extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions.
  • Post-translational
    modifications
    O-glycosylated.
    Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
  • Information by UniProt
  • Database links
  • Alternative names
    • 68 kDa neurofilament protein antibody
    • 68kDa Neurofilament antibody
    • 68kDa neurofilament protein antibody
    • CMT1F antibody
    • CMT2E antibody
    • FLJ53642 antibody
    • Light molecular weight neurofilament protein antibody
    • NEFL antibody
    • Neurofilament light antibody
    • Neurofilament light polypeptide 68kDa antibody
    • Neurofilament light polypeptide antibody
    • Neurofilament protein, light chain antibody
    • Neurofilament subunit NF L antibody
    • Neurofilament triplet L protein antibody
    • NF-L antibody
    • NF68 antibody
    • NFL antibody
    • NFL_HUMAN antibody
    see all

Images

  • Immunohistochemical analysis of Paraffin-embedded Human lung carcinoma tissue labelling 68kDa Neurofilament / NF-L with ab135884 at 1/50 dilution.

References

ab135884 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab135884.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up