Product nameAnti-68kDa Neurofilament / NF-L antibody
See all 68kDa Neurofilament / NF-L primary antibodies
DescriptionRabbit polyclonal to 68kDa Neurofilament / NF-L
SpecificitySpecifically recognizes the light neurofilament subunit (~68 kDa).
Tested applicationsSuitable for: WB, IHC-FoFr, IHC-P, IHC-Fr, ICC/IF, IHC-FrFlmore details
Species reactivityReacts with: Rat, Pig
Predicted to work with: Bird, Mammals
Full length native protein (purified) corresponding to Pig 68kDa Neurofilament/ NF-L. prepared from spinal cords by the method of Delacourte et al. and this cytoskeletal material was dissolved in 6M urea. Purified by ion exchange chromatography, then preparative gel electrophoresis.
Previously labelled as 68kDa Neurofilament.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Our Abpromise guarantee covers the use of ab9035 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionNeurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
Involvement in diseaseDefects in NEFL are the cause of Charcot-Marie-Tooth disease type 1F (CMT1F) [MIM:607734]. CMT1F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. CMT1F is characterized by onset in infancy or childhood (range 1 to 13 years).
Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 2E (CMT2E) [MIM:607684]. CMT2E is an autosomal dominant form of Charcot-Marie-Tooth disease type 2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy.
Sequence similaritiesBelongs to the intermediate filament family.
DomainThe extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions.
Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
- Information by UniProt
- 68 kDa neurofilament protein antibody
- 68kDa Neurofilament antibody
- 68kDa neurofilament protein antibody
Immunohistochemistry (Free Floating) analysis of mouse cerebellum staining 68kDa Neurofilament / NF-L with ab9035 (1/5000) in red. Costained with chicken pAb to MBP (1/5000) in green and DAPI in blue. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with above antibodies.
Lane 1 : Protein ladder
Lanes 2-5 : Anti-68kDa Neurofilament / NF-L antibody (ab9035) at 1/20000 dilution
Lane 2 : Rat brain
Lane 3 : Rat spinal cord
Lane 4 : Mouse brain
Lane 5 : Mouse spinal cord
Observed band size: 68 kDa why is the actual band size different from the predicted?
ab9035 staining anti 68kDa Neurofilament / NF-L in mixed neuron/glia cultures from newborn rat brain by ICC/IF (Immunocytochemistry/immunofluorescence). Samples were incubated with primary antibody (1/500) (red) and co-stained with ab4573 for anti Peripherin (green).
ab9035 at a 1/300 dilution staining rat hippocampal organotypic slice cultures by ICC/IF. The primary antibody was incubated with the cells for 120 hours (this time allows for the antibody to penetrate a layer of glial cells that grows while the slice is in culture). Bound antibody is detected using a Cy3 conjugated Indocarbocyanine.
This product has been referenced in:
- Yadav P et al. Neurofilament depletion improves microtubule dynamics via modulation of Stat3/stathmin signaling. Acta Neuropathol 132:93-110 (2016). WB ; Mouse . Read more (PubMed: 27021905) »
- Clarke WT et al. Syncoilin modulates peripherin filament networks and is necessary for large-calibre motor neurons. J Cell Sci : (2010). WB ; Mouse . Read more (PubMed: 20587592) »