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A preparation of enzymatically dephosphorylated pig neurofilaments including NF-L, NF-M and NF-H. Screening was by ELISA on the immungen followed by immunofluorescence microscopy.
Our Abpromise guarantee covers the use of ab7255 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/100 - 1/500.|
|WB||1/5000 - 1/10000.|
|IHC-FoFr||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. (works on mildly formalin fixed sections)|
|IHC-Fr||Use at an assay dependent concentration.|
Ab7255 staining Neurofilament in rat frontal cortex sections by Immunocytochemistry/Immunofluorescence. Samples were incubated with primary antibody at 1/5000 dilution (red) and chicken polyclonal to GFAP.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"