• Product name

    Anti-68kDa Neurofilament/NF-L antibody [2F11], prediluted
    See all 68kDa Neurofilament/NF-L primary antibodies
  • Description

    Mouse monoclonal [2F11] to 68kDa Neurofilament/NF-L, prediluted
  • Host species

  • Specificity

    ab74592 labels neurons, neuronal processes, and peripheral nerves as well as sympathetic ganglion cells and adrenal medulla. Cell body of neurons, containing the non-phosphorylated neurofilament, is weakly stained. We have data to indicate that this antibody may not cross react with Dog. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.

    Detects 68 kDa component of the polypeptide subunit. Some labs have reported an additional 200 kDa band under certain conditions. We cannot confirm if this is in fact a multimer of the 68 kDa protein.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Rabbit, Cat, Human
  • Immunogen

    Full length native protein (purified) corresponding to Human 68kDa Neurofilament/NF-L.

  • Positive control

    • Human and rat brain cerebellum tissue.
  • General notes

    In originator's published literature (e.g. PMID 6206727), this clone 2F11 has been shown specific to 68kDa protein however our testing on two different occasions show a 200 kDa protein band in western blot, under reduced and denaturing conditions. The 200 kDa could be either the glycosylated form of the 68kDa or 200kD a protein however we haven't done any further test to confirm it. We will welcome any feedback from researchers have who used the same clone.

    Previously labelled as 68kDa Neurofilament. 



Our Abpromise guarantee covers the use of ab74592 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
IHC-P 1/1.


  • Function

    Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
  • Involvement in disease

    Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 1F (CMT1F) [MIM:607734]. CMT1F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. CMT1F is characterized by onset in infancy or childhood (range 1 to 13 years).
    Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 2E (CMT2E) [MIM:607684]. CMT2E is an autosomal dominant form of Charcot-Marie-Tooth disease type 2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Domain

    The extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions.
  • Post-translational

    Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
  • Information by UniProt
  • Database links

  • Alternative names

    • 68 kDa neurofilament protein antibody
    • 68kDa Neurofilament antibody
    • 68kDa neurofilament protein antibody
    • CMT1F antibody
    • CMT2E antibody
    • FLJ53642 antibody
    • Light molecular weight neurofilament protein antibody
    • NEFL antibody
    • Neurofilament light antibody
    • Neurofilament light polypeptide 68kDa antibody
    • Neurofilament light polypeptide antibody
    • Neurofilament protein, light chain antibody
    • Neurofilament subunit NF L antibody
    • Neurofilament triplet L protein antibody
    • NF-L antibody
    • NF68 antibody
    • NFL antibody
    • NFL_HUMAN antibody
    see all


  • Immunohistochemical analysis of rat cerebellum tissue labeling Neurofilament with ab74592 using peroxidase-conjugated and AEC chromogen. 

  • Immunohistochemical analysis of human cerebellum tissue labeling Neurofilament with ab74592 using peroxidase-conjugated and DAB chromogen. 


ab74592 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A


Thank you for contacting us.

ab74592; This is to inform you that we have retested the product from the latest two bulk lots that we have, and using IMR5 cell lysate, we have detected 2 bands – 200 and around 70 kDa.

ab910: This antibody is designed for 68kDa protein however due to copolymer formation between 70kDa and 200kda protein it detects band at >200kDa as well.

ab8970: is indeed anti 70 kDa protein. The target has been hanged.

Coul dyou tell us, which datasheet say the ab detects 2kDa protein?

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Thanks you for your email. Apologies for delay.


This paper talks about how non producing cells can overtake a population and how it can potentially be resolved. It also mentions in the introduction how these changes may be due to mutation/loss of genes and cites some further references that may be useful.

These changes are likely to be what brings about lab-to-lab differences in productions of the same clone, particularly as the number of independent productions increases ie. The first production at each lab from a batch of identical cells received from the licensor is likely to be comparable but several productions later each lab may have slightly different populations of cells due to generally random nature of mutations/cell changes. Differences could potentially be further enhanced via subcloning and selection of a specific clone with enhanced traits (e.g. better secretion, better staining in IHC etc) for future productions.

I hope this infromaiton will be helpful.

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