Question (65645) | Anti-68kDa Neurofilament/NF-L antibody [2F11], prediluted (ab74592)

Go to datasheet (ab74592)

Question

I have a customer enquiry re the specificity of these NF-L antibodies: ab8970, ab910, ab74592

Customer’s enquiry:

“I am confused about the specificity of this Ab. Clone 2F11, marketed by other companies reacts with NF-L (68-70kD) only. Yours appears to react with both 68kD and 200kD neurofilaments?? Are these antibodies cocktails or is there some variation with the specificity?

“They also said “look at the Dako Ab.. also 2F11 clone.. and others (google 2F11).”

Your data sheet suggests that the antibody recognises the 64 and 200 kDa proteins, but they are separate entities – so it would be unlikely that a monoclonal would recognise both? Also, in one of the data sheet it says that it detects a 2kDa protein??

I look forward to your reply.

Answer

Thank you for contacting us.

ab74592; This is to inform you that we have retested the product from the latest two bulk lots that we have, and using IMR5 cell lysate, we have detected 2 bands – 200 and around 70 kDa.

ab910: This antibody is designed for 68kDa protein however due to copolymer formation between 70kDa and 200kda protein it detects band at >200kDa as well.

ab8970: is indeed anti 70 kDa protein. The target has been hanged.

Coul dyou tell us, which datasheet say the ab detects 2kDa protein?

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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