Key features and details
- Chicken polyclonal to 68kDa Neurofilament/NF-L
- Suitable for: IHC-FoFr, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgY
Product nameAnti-68kDa Neurofilament/NF-L antibody
See all 68kDa Neurofilament/NF-L primary antibodies
DescriptionChicken polyclonal to 68kDa Neurofilament/NF-L
Tested applicationsSuitable for: IHC-FoFr, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human 68kDa Neurofilament/NF-L conjugated to keyhole limpet haemocyanin. sequence shared between the Human (P07196) and Mouse (P08551). Made from three KLH conjugated synthetic peptides corresponding to different regions of NF-L.
General notesDo not freeze the antibody unless you want to store it for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half its binding activity is lost.
Previously labelled as 68kDa Neurofilament.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesIgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.3 mg/ml. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.
Our Abpromise guarantee covers the use of ab134460 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||1/1000 - 1/2000. Use 2% paraformaldehyde-fixed tissues.|
|ICC/IF||1/1000 - 1/2000. Use 2% paraformaldehyde-fixed cells.|
FunctionNeurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
Involvement in diseaseDefects in NEFL are the cause of Charcot-Marie-Tooth disease type 1F (CMT1F) [MIM:607734]. CMT1F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. CMT1F is characterized by onset in infancy or childhood (range 1 to 13 years).
Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 2E (CMT2E) [MIM:607684]. CMT2E is an autosomal dominant form of Charcot-Marie-Tooth disease type 2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy.
Sequence similaritiesBelongs to the intermediate filament family.
DomainThe extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions.
Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
- Information by UniProt
- 68 kDa neurofilament protein antibody
- 68kDa Neurofilament antibody
- 68kDa neurofilament protein antibody
Immunocytochemistry/Immunofluorescence analysis of dissociated adult mouse cerebral cortical cells labelling 68kDa Neurofilament/NF-L (green) with ab134460. Cells were fixed with 4% paraformaldehyde. Red - rat anti-NFM, Blue - DAPI nuclear stain.
Immunohistochemical analysis of adult mouse brain labelling 68kDa Neurofilament/NF-L in axons in the white matter of the cerebellum with ab134460 at 1/1000 dilution (brown staining).
Immunocytochemistry/Immunofluorescence analysis of dissociated adult mouse cerebral cortical cells labelling 68kDa Neurofilament/NF-L (green) with ab134460. Cells were fixed with 4% paraformaldehyde.
ab134460 has not yet been referenced specifically in any publications.