Overview

  • Product name

    Anti-68kDa Neurofilament/NF-L antibody
    See all 68kDa Neurofilament/NF-L primary antibodies
  • Description

    Chicken polyclonal to 68kDa Neurofilament/NF-L
  • Host species

    Chicken
  • Tested applications

    Suitable for: IHC-FoFr, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human 68kDa Neurofilament/NF-L conjugated to keyhole limpet haemocyanin. sequence shared between the Human (P07196) and Mouse (P08551). Made from three KLH conjugated synthetic peptides corresponding to different regions of NF-L.

  • Positive control

    • Adult mouse brain
  • General notes

    Do not freeze the antibody unless you want to store it for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half its binding activity is lost.

    Previously labelled as 68kDa Neurofilament. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 98% PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.3 mg/ml. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.
  • Clonality

    Polyclonal
  • Isotype

    IgY
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab134460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/1000 - 1/2000. Use 2% paraformaldehyde-fixed tissues.
WB 1/2000 - 1/5000. Predicted molecular weight: 62 kDa.
ICC/IF 1/1000 - 1/2000. Use 2% paraformaldehyde-fixed cells.

Target

  • Function

    Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
  • Involvement in disease

    Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 1F (CMT1F) [MIM:607734]. CMT1F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. CMT1F is characterized by onset in infancy or childhood (range 1 to 13 years).
    Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 2E (CMT2E) [MIM:607684]. CMT2E is an autosomal dominant form of Charcot-Marie-Tooth disease type 2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Domain

    The extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions.
  • Post-translational
    modifications

    O-glycosylated.
    Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
  • Information by UniProt
  • Database links

  • Alternative names

    • 68 kDa neurofilament protein antibody
    • 68kDa Neurofilament antibody
    • 68kDa neurofilament protein antibody
    • CMT1F antibody
    • CMT2E antibody
    • FLJ53642 antibody
    • Light molecular weight neurofilament protein antibody
    • NEFL antibody
    • Neurofilament light antibody
    • Neurofilament light polypeptide 68kDa antibody
    • Neurofilament light polypeptide antibody
    • Neurofilament protein, light chain antibody
    • Neurofilament subunit NF L antibody
    • Neurofilament triplet L protein antibody
    • NF-L antibody
    • NF68 antibody
    • NFL antibody
    • NFL_HUMAN antibody
    see all

Images

  • Immunocytochemistry/Immunofluorescence analysis of dissociated adult mouse cerebral cortical cells labelling 68kDa Neurofilament/NF-L (green) with ab134460. Cells were fixed with 4% paraformaldehyde. Red - rat anti-NFM, Blue - DAPI nuclear stain.

  • Immunohistochemical analysis of adult mouse brain labelling 68kDa Neurofilament/NF-L in axons in the white matter of the cerebellum with ab134460 at 1/1000 dilution (brown staining).

  • Immunocytochemistry/Immunofluorescence analysis of dissociated adult mouse cerebral cortical cells labelling 68kDa Neurofilament/NF-L (green) with ab134460. Cells were fixed with 4% paraformaldehyde.

References

ab134460 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Question
Answer

The immunogen used for ab134460 are three synthetic peptides conjugated to KLH, corresponding to different regions of the 68kDa Neurofilament gene product, but shared between the Human (P07196) and Mouse (P08551) protein sequences.

The first two peptides occur in N-terminal quarter of the protein (Peptide #1 between the 50th and 100th residue, Peptide #2, between the 100th and 150th residue). The last peptide is near center between residue 250 and 300. The last peptide is also near a coiled domain.

Read More

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