• Product name
    Anti-68kDa Neurofilament/NF-L antibody
    See all 68kDa Neurofilament/NF-L primary antibodies
  • Description
    Chicken polyclonal to 68kDa Neurofilament/NF-L
  • Host species
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, WB, ICC/IF, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Cow, Human
  • Immunogen

    Tissue, cells or virus corresponding to Cow 68kDa Neurofilament/NF-L.

  • Positive control
    • Rat cerebral cortex lysate. This antibody gave a positive result in IF/ICC when used in the following formaldehyde fixed cell lines: SKNSH.
  • General notes

    Previously labelled as 68kDa Neurofilament. 



Our Abpromise guarantee covers the use of ab72997 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
WB 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 61 kDa).
ICC/IF 1/5000.
ICC Use at an assay dependent concentration.


  • Function
    Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
  • Involvement in disease
    Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 1F (CMT1F) [MIM:607734]. CMT1F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. CMT1F is characterized by onset in infancy or childhood (range 1 to 13 years).
    Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 2E (CMT2E) [MIM:607684]. CMT2E is an autosomal dominant form of Charcot-Marie-Tooth disease type 2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy.
  • Sequence similarities
    Belongs to the intermediate filament family.
  • Domain
    The extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions.
  • Post-translational
    Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
  • Information by UniProt
  • Database links
  • Alternative names
    • 68 kDa neurofilament protein antibody
    • 68kDa Neurofilament antibody
    • 68kDa neurofilament protein antibody
    • CMT1F antibody
    • CMT2E antibody
    • FLJ53642 antibody
    • Light molecular weight neurofilament protein antibody
    • NEFL antibody
    • Neurofilament light antibody
    • Neurofilament light polypeptide 68kDa antibody
    • Neurofilament light polypeptide antibody
    • Neurofilament protein, light chain antibody
    • Neurofilament subunit NF L antibody
    • Neurofilament triplet L protein antibody
    • NF-L antibody
    • NF68 antibody
    • NFL antibody
    • NFL_HUMAN antibody
    see all


  • Anti-68kDa Neurofilament/NF-L antibody (ab72997) at 1/10000 dilution + Rat cerebral cortex lysate

    Predicted band size: 61 kDa
    Observed band size: 68 kDa
    why is the actual band size different from the predicted?

  • ab72997 staining 68kDa Neurofilament/NF-L in Mouse retina tissue by Immunohistochemistry (Frozen sections). Sections were fixed with formaldehyde and permeabilized in 0.3% Triton X-100 prior to blocking in 3% BSA for 1 hour at 25°C. ab72997 was diluted 1/500 and incubated with the sample for 4 hours at 4°C. An Alexa Fluor® 546-conjugated Goat anti-Chicken antibody, diluted 1/500 was used as the secondary antibody.

    See Abreview

  • ab72997 staining 68kDa Neurofilament/NF-L in rat 33B cells by Immunocytochemistry/ Immunofluorescence.

    Cells were fixed with paraformaldehyde and blocked with 10% serum for 20 minutes at 24°C. Samples were incubated with primary antibody (1/500 in PBS) for 16 hours at 4°C. An AlexaFluor®555-conjugated goat anti-chicken polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • ICC/IF image of ab72997 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab72997 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- chicken IgY (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab72997 staining 68kDa Neurofilament/NF-L in mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde for 1 hour and blocked with 10% goat serum for 30 minutes at room temperature. Samples were incubated with primary antibody (1/500 in PBS) for 16 hours at 4°C. An Alexa Fluor® 555-conjugated goat anti-chicken IgY polyclonal (1/1000) was used as the secondary antibody. Counterstained with Hoechst 33258 (blue) for 10 minutes at room temperature.

    See Abreview


This product has been referenced in:
  • Karbassi E  et al. Direct visualization of cardiac transcription factories reveals regulatory principles of nuclear architecture during pathological remodeling. J Mol Cell Cardiol 128:198-211 (2019). Read more (PubMed: 30742811) »
  • Clements MP  et al. The Wound Microenvironment Reprograms Schwann Cells to Invasive Mesenchymal-like Cells to Drive Peripheral Nerve Regeneration. Neuron 96:98-114.e7 (2017). IHC-Fr . Read more (PubMed: 28957681) »
See all 6 Publications for this product

Customer reviews and Q&As

1-3 of 3 Q&A


The concentration of your lot (GR124671-2) of ab72997 is 21.5 mg/ml.

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Thank you for your email.
The size difference will effect WB not IHC. However it will more depend on the tissues section you will be using.

Please check the localization of different chains in axons

I hope this info will be helpful.

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Thank you for contacting us. I am afraid we do not have exact match of monoclonal the customer is looking for. We however have many antibodies that are against the neurofilament proteins that forms part of Axons. As by definition Neurofilaments (NFs) are the major structural proteins of neurons. They are most abundant in larger neurons and are heavily concentrated in axons, in particular long projection axons so these proteins can be used as markers for axons. The antibodies anti neurofilament antibodies we have are; ab7255, ab9035, ab88043, ab72997, ab3966 etc. https://www.abcam.com/index.html?pageconfig=searchresults&startPage=2 Please have a look at the select the antibody as per your interest. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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