Immunohistochemistry (Frozen sections) abreview for Anti-68kDa Neurofilament/NF-L antibody

Excellent
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Retina)
Permeabilization
Yes - 0.3% Triton X100
Specification
Retina
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS+3% BSA+ 0.3% TritonX100
Dilution
1/500

Secondary antibody

Dilution
1/500
Name
Non-Abcam antibody was used: Molecular Probes anti-chicken
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 546

Additional data

Additional Notes
This worked best for me with gentle PFA fixation 2% for about 30 minutes. Signal is strong, background is fairly minimal.

Ms. Catherine Sheely

Verified customer

Submitted Nov 16 2010

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