Immunocytochemistry/ Immunofluorescence abreview for Anti-68kDa Neurofilament/NF-L antibody

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (33B)
Permeabilization
No
Specification
33B
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS
Dilution
1/500

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: Invitrogen Goat Anti Chicken
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555

Additional data

Additional Notes
33B Neuroblastoma cell line was grown on cover slips in 6 well plates in DMEM/10% FCS. Cells were washed with PBS prior to fixing with 1%PFA. Fixed in 1%PFA prior to staining. Wash 3x3min in PBS. Block 10% goat serum for 20min at RT. Primary Ab diluted in PBS and incubated overnight at 4deg. Wash 3x3min in PBS. Secondary - goat anti-chicken AF555 (red) 1/1000 in PBS for 1h at RT. Wash 3x3min in PBS Counterstained with Hoechst 33258 (blue) for 10mins at RT. Wash 3min in dH2O Coverslips removed tapped dry and placed on glass slides and mounted in Invitrogen ProLong gold and kept at 4deg until imaged.

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Submitted Aug 01 2012

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