Western blot abreview for Anti-68kDa Neurofilament/NF-L antibody

Good
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse brain lysate)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
25 µg
Specification
Mouse brain lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk tbst
Dilution
1/10000

Secondary antibody

Dilution
1/100000
Name
Non-Abcam antibody was used: Sigma Anti chicken IgY
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
Ecl plus
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 68 kDa
Positive control
This sample

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Submitted Oct 24 2012

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