Immunohistochemistry (Frozen sections) abreview for Anti-68kDa Neurofilament / NF-L antibody

Good
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
No
Specification
Brain
Blocking step
(agent) for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS
Dilution
1/500

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: Invitrogen Goat anti Chicken AF555
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555

Additional data

Additional Notes
10um frozen section. Tissue fixed for 1h in 4%PFA, then 20% sucrose for 1h both at RT prior to embedding. Wash 3x3min in PBS. Block 10% goat serum for 30min at RT. Primary Ab diluted 1/500 in PBS and incubated overnight at 4deg, also works by incubating for 2 hours at RT. Wash 3x3min in PBS. Secondary - goat anti-chicken AF555 (red) 1/1000 in PBS for 1h at RT. Wash 3x3min in PBS Counterstained with Hoechst 33258 (blue) for 10mins at RT. Wash 3min in dH2O Mounted in Invitrogen ProLong gold and kept at 4deg until imaged.

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Submitted May 16 2014

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