Immunohistochemistry (Frozen sections) abreview for Anti-68kDa Neurofilament / NF-L antibody

Good
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Retina)
Permeabilization
Yes - 0.3% Triton X
Specification
Retina
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Fixative
Paraformaldehyde

Other product details

Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/500

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: Thermo Scientific Dylight 594 Goat anti chicken
Host species: Goat
Clonality: Polyclonal
Conjugation: DyLight® 594

Additional data

Additional Notes
Intense staining of the nerve fiber layer and ganglion cell layer. Counterstain with DAPI. Image taken @ 40x.

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Submitted Aug 19 2014

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