Biotin Anti-6X His tag® antibody [33D10.D2] (ab106261)
Key features and details
- Biotin Mouse monoclonal [33D10.D2] to 6X His tag®
- Suitable for: WB, ELISA
- Reacts with: Species independent
- Conjugation: Biotin
- Isotype: IgG1
Overview
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Product name
Biotin Anti-6X His tag® antibody [33D10.D2]
See all 6X His tag® primary antibodies -
Description
Biotin Mouse monoclonal [33D10.D2] to 6X His tag® -
Host species
Mouse -
Conjugation
Biotin -
Specificity
ab106261 reacts to over-expressed proteins containing the 6X His tag® fused to either the amino or carboxy terminus of transfected or transformed cells. To date, this antibody has reacted with all His tagged proteins so far tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.
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Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
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General notes
10-20 BAC molecules per Mouse Monoclonal Antibody
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.42% Tripotassium orthophosphate, 0.88% Sodium chloride -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
33D10.D2 -
Isotype
IgG1 -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106261 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 1 kDa.
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ELISA |
1/200000.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 1 kDa. |
ELISA
1/200000. |
Target
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Relevance
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. His-tags can be fused to the amino- or carboxy- termini of proteins in transfected or transformed cells. -
Cellular localization
Depends upon the localization of the parent protein tagged with hexahistidine. -
Alternative names
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
Images
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All lanes : Biotin Anti-6X His tag® antibody [33D10.D2] (ab106261) at 1/5000 dilution (overnight at 4°C)
Lane 1 : Purified histidine-tagged recombinant protein at 0.1 µg
Lane 2 : E. coli cell lysate containing histidine-tagged expression construct at 0.2 µg
Lane 3 : Purified GST-tagged recombinant protein at 0.1 µg
Lane 4 : Purified FLAG-tagged recombinant protein at 0.1 µg
Secondary
All lanes : Peroxidase mouse secondary antibody for 30 minutes at room temperature at 1/20000 dilution
Predicted band size: 1 kDaBlock: 5% non-fat powdered milk for 1 hour at room temperature.
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ELISA analysis of ab106261 Mouse monoclonal [33D10.D2] to 6X His tag® (Biotin).
Antigen: HIS-tagged purified protein and E. coli cell lysates expressing HIS-Tagged construct, GST- and RON-tagged purified proteins.
Coating amount: 0.15 µg per welll.
Primary antibody: ab106261 at 100 µg/mL.
Dilution series: 2-fold.
Mid-point concentration: 200 ng/mL.
Secondary antibody: Peroxidase conjugated mouse secondary antibody at 1/10000.
Substrate: TMB ELISA Substrate.
Datasheets and documents
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Datasheet download
References (0)
ab106261 has not yet been referenced specifically in any publications.