Overview

  • Product name

    Anti-6X His tag® antibody [33D10.D2] (Biotin)
    See all 6X His tag® primary antibodies
  • Description

    Mouse monoclonal [33D10.D2] to 6X His tag® (Biotin)
  • Host species

    Mouse
  • Conjugation

    Biotin
  • Specificity

    ab106261 detects over-expressed proteins containing the 6X His epitope tag. To date, this antibody has reacted with all His tagged proteins so far tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins. The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino or carboxy-termini of targeted proteins in transfected or transformed cells.
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.

  • General notes

    10-20 BAC molecules per Mouse Monoclonal Antibody

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 1% BSA, 0.42% Potassium phosphate, 0.88% Sodium chloride
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    33D10.D2
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab106261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 1 kDa.
ELISA 1/200000.

Target

  • Relevance

    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • Cellular localization

    Depends upon the localization of the parent protein tagged with hexahistidine.
  • Alternative names

    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

References

ab106261 has not yet been referenced specifically in any publications.

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