Mouse monoclonal [33D10.D2] to 6X His tag® (Biotin)
ab106261 detects over-expressed proteins containing the 6X His
epitope tag. To date, this antibody has reacted with all His tagged
proteins so far tested. In western blotting of bacterial extracts, the
antibody does not cross-react with endogenous proteins. The antibody
recognizes the His-tag (His-His-His-His-His-His) fused to either the amino or carboxy-termini of targeted proteins in transfected or transformed cells.
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Depends upon the localization of the parent protein tagged with hexahistidine.