Recombinant
Recombinant Anti-6X His tag® antibody [3D5] - C-terminal (ab206500)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-6X His tag® antibody [3D5] - C-terminal
See all 6X His tag® primary antibodies -
Description
Mouse monoclonal [3D5] to 6X His tag® - C-terminal -
Host species
Mouse -
Specificity
ab206500 is specific for carboxy-terminal oligo-histidine tags (6x His tags) of fusion proteins. -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Recombinant fragment corresponding to 6X His tag®. (Purified his tagged Fab fragment).
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General notes
ab206500 is a recombinant chimeric antibody manufactured with variable regions (i.e. specificity) from the hybridoma 3D5, created for improved compatibility with existing reagents, assays and techniques.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Proclin
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
3D5 -
Isotype
IgG2c -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab206500 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. | |
| ELISA | Use at an assay dependent concentration. |
Target
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Relevance
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. -
Cellular localization
Depends upon the localization of the parent protein tagged with hexahistidine. -
Alternative names
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
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Protocols
References
ab206500 has not yet been referenced specifically in any publications.
