Recombinant Anti-6X His tag® antibody [3D5] - C-terminal (ab206500)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [3D5] to 6X His tag® - C-terminal
- Suitable for: WB, ELISA
- Reacts with: Species independent
Overview
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Product name
Anti-6X His tag® antibody [3D5] - C-terminal
See all 6X His tag® primary antibodies -
Description
Mouse monoclonal [3D5] to 6X His tag® - C-terminal -
Host species
Mouse -
Specificity
ab206500 reacts to recombinant proteins containing the 6X His tag® fused to carboxy terminus of fusion proteins. It also has affinity for internal 6X His tag® but no affinity for 6X His tag® fused to amino terminus.
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Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Recombinant fragment corresponding to 6X His tag®. (Purified his tagged Fab fragment).
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General notes
ab206500 is a recombinant chimeric antibody manufactured with variable regions (i.e. specificity) from the hybridoma 3D5, created for improved compatibility with existing reagents, assays and techniques.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Proclin 300
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
3D5 -
Isotype
IgG2c -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab206500 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. | |
| ELISA | Use at an assay dependent concentration. |
Target
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Relevance
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. -
Cellular localization
Depends upon the localization of the parent protein tagged with hexahistidine. -
Alternative names
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
Images
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![Anti-6X His tag® antibody [3D5] - C-terminal (ab206500)](https://www.abcam.com/ps/products/206/ab206500/Images/ab206500-1-benefits-of-recombinant-antibodies.png)
Anti-6X His tag® antibody [3D5] - C-terminal (ab206500)
References (0)
ab206500 has not yet been referenced specifically in any publications.
