Recombinant Anti-6X His tag® antibody [3D5] - C-terminal (ab206504)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [3D5] to 6X His tag® - C-terminal
- Suitable for: ELISA, WB
- Reacts with: Species independent
Overview
-
Product name
Anti-6X His tag® antibody [3D5] - C-terminal
See all 6X His tag® primary antibodies -
Description
Rat monoclonal [3D5] to 6X His tag® - C-terminal -
Host species
Rat -
Specificity
ab206504 reacts to recombinant proteins containing the 6X His tag® fused to carboxy terminus of fusion proteins. It also has affinity for internal 6X His tag® but no affinity for 6X His tag® fused to amino terminus.
-
Tested applications
Suitable for: ELISA, WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Recombinant fragment corresponding to 6X His tag®. Purified His tagged Fab fragment.
-
General notes
ab206504 is a chimeric antibody created for improved compatibility with existing reagents, assays and techniques.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Proclin 300
Constituent: 99% PBS -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
3D5 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab206504 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ELISA |
Use at an assay dependent concentration.
|
|
WB |
Use at an assay dependent concentration.
|
Notes |
---|
ELISA
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
Target
-
Relevance
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. His-tags can be fused to the amino- or carboxy- termini of proteins in transfected or transformed cells. -
Cellular localization
Depends upon the localization of the parent protein tagged with hexahistidine. -
Alternative names
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab206504 has been referenced in 2 publications.
- Rocchi A et al. Autoantibodies to synapsin I sequestrate synapsin I and alter synaptic function. Cell Death Dis 10:864 (2019). PubMed: 31727880
- Lindner P et al. Specific detection of his-tagged proteins with recombinant anti-His tag scFv-phosphatase or scFv-phage fusions. Biotechniques 22:140-9 (1997). PubMed: 8994661