Western blot abreview for Anti-6X His tag® antibody [4D11]

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK 293 cells transfected with Beta3-His (CACNB3))
Loading amount
25 µg
Specification
HEK 293 cells transfected with Beta3-His (CACNB3)
Gel Running Conditions
Reduced Denaturing (10% SDS)
Blocking step
Milk as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/2000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1X PBS/Tween, 5% milk

Secondary antibody

Name
Non-Abcam antibody was used: anti mouse HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL+
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 55 kDa Non-specific: 85, 100, 130 kDa
Negative control
HEK-293 cells lysate

Additional data

Additional Notes
Line 1: HEK-293 lysate
Line 2: HEK-293 cells transfected with Beta3-His (CACNB3)

Herr Dr. Vladimir Milenkovic

Verified customer

Submitted Nov 20 2009

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