Immunoprecipitation abreview for Anti-6X His tag® antibody [4D11]

Good
Abreviews
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HEK 293 cells transfected with Beta3-His (CACNB3))
Total protein in input
25 µg
Specification
HEK 293 cells transfected with Beta3-His (CACNB3)
Immuno-precipitation step
Protein G

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1%TX-100, 10% glycerol, 1XPBS

Western Blot antibody

Name
Non-Abcam antibody was used: anti rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Additional data

Additional Notes
Line 1. Lysate of HEK293 cells expressing beta3-His (CACNB3)
Line 2. IP with anti His Ab
Line 3. Not bound fraction
Beta3-His fusion protein was immunoprecipitated using anti His Ab, and specific beta3a band (56kDa) was detected using anti beta3 Ab (Alomone Labs).

Herr Dr. Vladimir Milenkovic

Verified customer

Submitted Nov 20 2009

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