Immunocytochemistry/ Immunofluorescence abreview for Anti-6X His tag® antibody [4D11]

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK 293 cells transfected with CACNB3-His)
Specification
HEK 293 cells transfected with CACNB3-His
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% TX100
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/250
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% goat serum, 0.1%TX100, 1XPBS

Secondary antibody

Name
Non-Abcam antibody was used: anti mouse Alexa Fluor 546
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 546
Dilution
1/500

Additional data

Additional Notes
HEK 293 cells were transfected wirh CACNB3-His and stained with anti His Ab (red) 36 h after transfection. DAPI was used for nuclei staining (blue).

Herr Dr. Vladimir Milenkovic

Verified customer

Submitted Nov 20 2009

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