Western blot abreview for Anti-6X His tag® antibody [4D11]

Good
Abreviews
Application
Western blot
Sample
Human Recombinant protein (recombinant protein (human))
Loading amount
20 µg
Specification
recombinant protein (human)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Other product details

Dilution
1/2000
Incubation time
24 hour(s) and 0 minute(s)

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti mouse HRP
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 80 kDa

Additional data

Additional Notes
I used this antibody to detect recombinant His-tagged DSC-1 and it worked quite well. I would recommend it to anyone who is interested in confirming recombinant His-tagged protein expression.

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Verified customer

Submitted Nov 27 2006

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