Overview

  • Product name

    Anti-6X His tag® antibody
    See all 6X His tag® primary antibodies
  • Description

    Rabbit polyclonal to 6X His tag®
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    A synthetic peptide: HHHHHH (6-His) conjugated to KLH.

  • Positive control

    • ab2431 E. coli Positive Control (Escherichia coli) Whole Cell Lysate - expressing 6X His tag protein. His-tagged recombinant proteins.
  • General notes

    HIS-TAG® is a trademark of EMD Biosciences, Inc.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab137839 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000.
IP 1/500.

Target

  • Relevance

    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • Cellular localization

    Depends upon the localization of the parent protein tagged with hexahistidine.
  • Alternative names

    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

Images

  • All lanes : Anti-6X His tag® antibody (ab137839) at 1/1000 dilution

    Lane 1 : Non-transformed E. Coli lysate
    Lane 2 : Non-transformed E. Coli lysate, induced
    Lane 3 : E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), non-induced
    Lane 4 : E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), induced

    Lysates/proteins at 10 µg per lane.


    12% SDS PAGE
  • All lanes : Anti-6X His tag® antibody (ab137839)

    Lane 1 : E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), non-induced
    Lane 2 : E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), induced

    Lysates/proteins at 10 µg per lane.
  • His-tagged protein was immunoprecipitated from transfected cells using 8 ug of ab137839. The precipitated His-tagged protein was detected by ab137839 diluted at 1/500.

References

This product has been referenced in:

  • Rudan M  et al. Normal mitochondrial function in Saccharomyces cerevisiae has become dependent on inefficient splicing. Elife 7:N/A (2018). Read more (PubMed: 29570052) »
  • Pearson CS  et al. Identification of a critical sulfation in chondroitin that inhibits axonal regeneration. Elife 7:N/A (2018). Read more (PubMed: 29762123) »
See all 7 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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