Anti-6X His tag® antibody (ab14923)
Key features and details
- Rabbit polyclonal to 6X His tag®
- Suitable for: WB
- Reacts with: Species independent
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-6X His tag® antibody
See all 6X His tag® primary antibodies -
Description
Rabbit polyclonal to 6X His tag® -
Host species
Rabbit -
Specificity
ab14923 reacts to recombinant proteins containing the 6X His tag® fused to either the amino or carboxy terminus in transfected or transformed cells.
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Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
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Positive control
- E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein, ab2431.
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General notes
HIS-TAG® is a trademark of EMD Biosciences, Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab14923 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 0.5 µg/ml.
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Notes |
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WB
Use a concentration of 0.5 µg/ml. |
Target
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Relevance
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. His-tags can be fused to the amino- or carboxy- termini of proteins in transfected or transformed cells. -
Cellular localization
Depends upon the localization of the parent protein tagged with hexahistidine. -
Alternative names
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
Images
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Anti-6X His tag® antibody (ab14923) at 0.6 µg/ml +
E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein (ab2431) at 10 µg
Secondary
Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution
Performed under reducing conditions.
Observed band size: 17 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab14923 has been referenced in 15 publications.
- Wan J et al. MYB70 modulates seed germination and root system development in Arabidopsis. iScience 24:103228 (2021). PubMed: 34746697
- Zhang X et al. SIZ1-Mediated SUMO Modification of SEUSS Regulates Photomorphogenesis in Arabidopsis. Plant Commun 1:100080 (2020). PubMed: 33367258
- Yang L et al. PIF1 and RVE1 form a transcriptional feedback loop to control light-mediated seed germination in Arabidopsis. J Integr Plant Biol 62:1372-1384 (2020). PubMed: 32277734
- Yang L et al. Interplay between REVEILLE1 and RGA-LIKE2 regulates seed dormancy and germination in Arabidopsis. New Phytol 225:1593-1605 (2020). PubMed: 31580487
- Salari S et al. Evaluation of a new live recombinant vaccine against cutaneous leishmaniasis in BALB/c mice. Parasit Vectors 13:415 (2020). PubMed: 32787908