Anti-6X His tag® antibody (ab9107)
Key features and details
- Chicken polyclonal to 6X His tag®
- Suitable for: WB, ELISA
- Reacts with: Species independent
- Isotype: unknown
Overview
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Product name
Anti-6X His tag® antibody
See all 6X His tag® primary antibodies -
Description
Chicken polyclonal to 6X His tag® -
Host species
Chicken -
Specificity
Antibody specificity was verified by ELISA against peptide conjugated to BSA (HHHHHH-BSA). A 1:8000 dilution of the antibody gave an OD=1.0 in a 15 minute reaction using Goat Anti Chicken IgY (IgG) and TMB as the substrate. Appropriate specificity controls were run. -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide corresponding to 6X His tag® conjugated to keyhole limpet haemocyanin.
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General notes
HIS-TAG® is a trademark of EMD Biosciences, Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.1% Sodium azide -
Concentration information loading...
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Purity
IgY fraction -
Purification notes
Antibodies were immunoaffinity purified using the peptide immobilized on a solid support. -
Clonality
Polyclonal -
Isotype
unknown -
Light chain type
unknown -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9107 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 1 kDa.
This antibody was an effective at 1:1000 (1 µg/ml) dilution using an affinity purified goat anti chicken IgY (IgG) HRP. The Westerns probed with this antibody are very sharp and clear with extremely low background and no non-specific binding. |
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ELISA |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 1 kDa. This antibody was an effective at 1:1000 (1 µg/ml) dilution using an affinity purified goat anti chicken IgY (IgG) HRP. The Westerns probed with this antibody are very sharp and clear with extremely low background and no non-specific binding. |
ELISA
Use at an assay dependent concentration. |
Target
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Relevance
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. His-tags can be fused to the amino- or carboxy- termini of proteins in transfected or transformed cells. -
Cellular localization
Depends upon the localization of the parent protein tagged with hexahistidine. -
Alternative names
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab9107 has not yet been referenced specifically in any publications.