Key features and details
- Chicken polyclonal to 6X His tag®
- Suitable for: WB, ELISA
- Reacts with: Species independent
- Isotype: unknown
Product nameAnti-6X His tag® antibody
See all 6X His tag® primary antibodies
DescriptionChicken polyclonal to 6X His tag®
SpecificityAntibody specificity was verified by ELISA against peptide conjugated to BSA (HHHHHH-BSA). A 1:8000 dilution of the antibody gave an OD=1.0 in a 15 minute reaction using Goat Anti Chicken IgY (IgG) and TMB as the substrate. Appropriate specificity controls were run.
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Species independent
Synthetic peptide: HHHHHH conjugated to KLH.
General notesHIS-TAG® is a trademark of EMD Biosciences, Inc.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.1% Sodium azide
Concentration information loading...
Purification notesAntibodies were immunoaffinity purified using the peptide immobilized on a solid support.
Light chain typeunknown
Our Abpromise guarantee covers the use of ab9107 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The Westerns probed with this antibody are very sharp and clear with extremely low background and no non-specific binding.
RelevanceThe H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Cellular localizationDepends upon the localization of the parent protein tagged with hexahistidine.
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
ab9107 has not yet been referenced specifically in any publications.