Product nameAnti-6X His tag® antibody
See all 6X His tag® primary antibodies
DescriptionGoat polyclonal to 6X His tag®
This antibody was verified by ELISA against peptide conjugated to BSA(HHHHHH/BSA).
This antibody does not detect all N-terminal 6-His tagged proteins.
Tested applicationsSuitable for: ELISA, ICC/IF, ICC, WBmore details
Species reactivityReacts with: Species independent
General notesHIS-TAG® is a trademark of EMD Biosciences, Inc.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at280 nm of 1.4 equals 1.0 mg of IgG.
Our Abpromise guarantee covers the use of ab9136 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/100 - 1/500.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 22247289|
|ICC||1/200 - 1/2000.|
|WB||1/1000 - 1/5000.|
RelevanceThe H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Cellular localizationDepends upon the localization of the parent protein tagged with hexahistidine.
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
ab9136, staining 6X His tag® in VMP001-conjugated nanoparticles by Immunocytochemistry/ Immunofluorescence. An AlexaFluor®488-conjugated anti-goat IgG was used as the secondary antibody.
All lanes : Anti-6X His tag® antibody (ab9136) at 1/5000 dilution
Lane 1 : E.coli whole cell lysate expressing a multi-tag fusion protein at 0.2 µg
Lane 2 : E.coli whole cell lysate expressing a multi-tag fusion protein at 0.1 µg
Lane 3 : E.coli whole cell lysate expressing a multi-tag fusion protein at 0.05 µg
Developed using the ECL technique.
Exposure time: 3 minutes
This product has been referenced in:
- Thapa P et al. Histone deacetylase 3 is required for iNKT cell development. Sci Rep 7:5784 (2017). Read more (PubMed: 28724935) »
- Unger A et al. Translocation of molecular chaperones to the titin springs is common in skeletal myopathy patients and affects sarcomere function. Acta Neuropathol Commun 5:72 (2017). Read more (PubMed: 28915917) »