Anti-6X His tag® antibody [AD1.1.10] (ab15149)

Submit a review Q&A (2)References (7)
Western blot - Anti-6X His tag® antibody [AD1.1.10] (ab15149)

    Key features and details

    • Mouse monoclonal [AD1.1.10] to 6X His tag®
    • Suitable for: ICC/IF, IHC-Fr, IP, WB
    • Reacts with: Species independent
    • Isotype: IgG1

    Conjugates logo Related conjugates and formulations

    Alkaline Phosphatase Biotin DyLight® 488 DyLight® 550 DyLight® 650 Europium HRP PE PerCP SureLight® APC SureLight® APC SureLight® P3

    Overview

    • Product name

      Anti-6X His tag® antibody [AD1.1.10]
      See all 6X His tag® primary antibodies

    • Description

      Mouse monoclonal [AD1.1.10] to 6X His tag®

    • Host species

      Mouse

    • Tested applications

      Suitable for: ICC/IF, IHC-Fr, IP, WBmore details

    • Species reactivity

      Reacts with: Species independent

    • Immunogen

      PAX6 transcription factor linked to histidine tag.

    • General notes

      HIS-TAG® is a trademark of EMD Biosciences, Inc.

       

      ab15149 recognises proteins containing the motif HHHHHH and is therefore of value in detecting proteins containing histidine tags. The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells.

      The clone number has been updated from (3H2201) to (AD1.1.10) both clone numbers name the same antibody clone.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • Storage buffer

      pH: 7.20
      Preservative: 0.01% Sodium azide
      Constituents: 0.58% Sodium chloride, 95% Sodium phosphate
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Purification notes

      Tissue cuture supernatant purified by Protein G affinity chromatography.

    • Primary antibody notes

      ab15149 recognises proteins containing the motif HHHHHH and is therefore of value in detecting proteins containing histidine tags. The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells.

    • Clonality

      Monoclonal

    • Clone number

      AD1.1.10

    • Myeloma

      NS1

    • Isotype

      IgG1

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab15149 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    IP
    Use at an assay dependent concentration.
    WB
    1/500 - 1/100.
    Notes
    ICC/IF
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    IP
    Use at an assay dependent concentration.
    WB
    1/500 - 1/100.

    Target

    • Relevance

      The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

    • Cellular localization

      Depends upon the localization of the parent protein tagged with hexahistidine.

    • Alternative names

      • 6 His epitope tag antibody
      • Hexa His tag antibody
      • HHHHHH epitope tag antibody
      • HHHHHH tag antibody
      • His tag antibody
      • Polyhistidine Tag antibody
      see all

    Images

    • Western blot - Anti-6X His tag® antibody [AD1.1.10] (ab15149)
      Western blot - Anti-6X His tag® antibody [AD1.1.10] (ab15149)
      All lanes : Anti-6X His tag® antibody [AD1.1.10] (ab15149) at 0.5 µg/ml

      Lane 1 : 60 kDa 6X His tagged protein at 0.3 µg
      Lane 2 : 60 kDa 6X His tagged protein at 0.1 µg
      Lane 3 : 60 kDa 6X His tagged protein at 0.03 µg
      Lane 4 : 60 kDa 6X His tagged protein at 0.01 µg
      Lane 5 : 60 kDa 6X His tagged protein at 0.003 µg

      Secondary
      All lanes : Goat Anti-Mouse (HRP) at 0.04 µg/ml


      Imaged using West Pico chemiluminescent substrate.

    References (7)

    Publishing research using ab15149? Please let us know so that we can cite the reference in this datasheet.

    ab15149 has been referenced in 7 publications.

    • Yi KS  et al. Broader neutralization of CT-P27 against influenza A subtypes by combining two human monoclonal antibodies. PLoS One 15:e0236172 (2020). PubMed: 32726321
    • Lam M  et al. Misfolded proteins bind and activate death receptor 5 to trigger apoptosis during unresolved endoplasmic reticulum stress. Elife 9:N/A (2020). PubMed: 31904339
    • Moreland KT  et al. In vitro calcite crystal morphology is modulated by otoconial proteins otolin-1 and otoconin-90. PLoS One 9:e95333 (2014). WB . PubMed: 24748133
    • Smith MH  et al. Misfolded proteins induce aggregation of the lectin Yos9. J Biol Chem 289:25670-7 (2014). WB . PubMed: 25086047
    • Ma L  et al. Serum anti-CCNY autoantibody is an independent prognosis indicator for postoperative patients with early-stage nonsmall-cell lung carcinoma. Dis Markers 35:317-25 (2013). WB . PubMed: 24167380
    • Ma Z  et al. Targeting human epidermal growth factor receptor signaling with the neuregulin's heparin-binding domain. J Biol Chem 284:32108-15 (2009). ICC/IF . PubMed: 19717564
    • Bergman LM  et al. Role of the unique N-terminal domain of CtBP2 in determining the subcellular localisation of CtBP family proteins. BMC Cell Biol 7:35 (2006). PubMed: 16999872

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Question

    I was looking for an anti-His-tag antibody. I
    found that in the description in some of them (ab5000, ab18184) it is
    indicated that the antibody recognizes recombinant protein expressed
    in mammalian cells and in some other (ab15149, ab81663) it is
    specified that the antibody recognizes protein expressed in bacteria.
    If the antibody is raised against His-tag epitope can it recognize
    indifferently recombinant protein expressed either in bacteria either
    in mammalian or eukariotic cells? or not?
    I had some problem with an anti-His-tag antibody of another company
    since recognize the protein expressed in bacteria but not in mammalian
    in WB and I would like to understand the reason before to look for a
    product from a different company.

    Read More
    Answer

    I can confirm that all four products, the ab5000, ab15149, ab18184 and ab81663 detect proteins, which contain either an N or C-terminal HHHHHHGS (6x) epitope tag.

    Important aspects for his tag recognition are, the localisation of the tag (N- or C-terminal) and the length of the his tag, that should be identical with the antibody chosen.

    Furthermore it is important to confirm that the tagged protein is expressed in transfected or transformed cells. If the recombinant protein is expressed, it should not make a difference for the detection if either a bacteria or a mammalian system was used.

    However, some his tag antibodies could cross react with other proteins in the used system. For ab81663, ab15149 there may be seen some cross reactivity with extracts of insect or mammalian cells

    Read More

    Question

    1.does ab15149 recognize n terminal histidine tags ? 2. If so does require just 6x His or 6xHis plus something else at the n terminal for the recognition ?

    Read More
    Answer

    This antibody recognises proteins containing the motif HHHHHH (5x His) and it is therefore of value in detecting proteins containing histidine tags (either at the N- or C-terminal). The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells.

    Read More

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