Anti-6X His tag® antibody [AD1.1.10] (ab15149)
Key features and details
- Mouse monoclonal [AD1.1.10] to 6X His tag®
- Suitable for: ICC/IF, IHC-Fr, IP, WB
- Reacts with: Species independent
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Anti-6X His tag® antibody [AD1.1.10]
See all 6X His tag® primary antibodies -
Description
Mouse monoclonal [AD1.1.10] to 6X His tag® -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, IHC-Fr, IP, WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
The clone number has been updated from (3H2201) to (AD1.1.10) both clone numbers name the same antibody clone.
HIS-TAG® is a trademark of EMD Biosciences, Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.58% Sodium chloride, 95% Sodium phosphate -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Tissue cuture supernatant purified by Protein G affinity chromatography. -
Primary antibody notes
ab15149 recognises proteins containing the motif HHHHHH and is therefore of value in detecting proteins containing histidine tags. The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells. -
Clonality
Monoclonal -
Clone number
AD1.1.10 -
Myeloma
NS1 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
- PerCP Anti-6X His tag® antibody [AD1.1.10] (ab117496)
- DyLight® 650 Anti-6X His tag® antibody [AD1.1.10] (ab117504)
- DyLight® 550 Anti-6X His tag® antibody [AD1.1.10] (ab117507)
- DyLight® 488 Anti-6X His tag® antibody [AD1.1.10] (ab117512)
- Biotin Anti-6X His tag® antibody [AD1.1.10] (ab15145)
- HRP Anti-6X His tag® antibody [AD1.1.10] (ab178563)
- Europium Anti-6X His tag® antibody [AD1.1.10] (ab187912)
- PE Anti-6X His tag® antibody [AD1.1.10] (ab72467)
- SureLight® APC Anti-6X His tag® antibody [AD1.1.10] (ab72579)
- Alkaline Phosphatase Anti-6X His tag® antibody [AD1.1.10] (ab81652)
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab15149 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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WB |
1/100 - 1/500.
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Notes |
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ICC/IF
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
WB
1/100 - 1/500. |
Target
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Relevance
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. His-tags can be fused to the amino- or carboxy- termini of proteins in transfected or transformed cells. -
Cellular localization
Depends upon the localization of the parent protein tagged with hexahistidine. -
Alternative names
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
Images
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All lanes : Anti-6X His tag® antibody [AD1.1.10] (ab15149) at 0.5 µg/ml
Lane 1 : 60 kDa 6X His tagged protein at 0.3 µg
Lane 2 : 60 kDa 6X His tagged protein at 0.1 µg
Lane 3 : 60 kDa 6X His tagged protein at 0.03 µg
Lane 4 : 60 kDa 6X His tagged protein at 0.01 µg
Lane 5 : 60 kDa 6X His tagged protein at 0.003 µg
Secondary
All lanes : Goat Anti-Mouse (HRP) at 0.04 µg/mlImaged using West Pico chemiluminescent substrate.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab15149 has been referenced in 9 publications.
- Ling H et al. A sugarcane smut fungus effector simulates the host endogenous elicitor peptide to suppress plant immunity. New Phytol 233:919-933 (2022). PubMed: 34716592
- Kishimoto H et al. The lipophilic cyclic peptide cyclosporin A induces aggregation of gel-forming mucins. Sci Rep 12:6153 (2022). PubMed: 35418571
- Yi KS et al. Broader neutralization of CT-P27 against influenza A subtypes by combining two human monoclonal antibodies. PLoS One 15:e0236172 (2020). PubMed: 32726321
- Lam M et al. Misfolded proteins bind and activate death receptor 5 to trigger apoptosis during unresolved endoplasmic reticulum stress. Elife 9:N/A (2020). PubMed: 31904339
- Moreland KT et al. In vitro calcite crystal morphology is modulated by otoconial proteins otolin-1 and otoconin-90. PLoS One 9:e95333 (2014). WB . PubMed: 24748133
- Smith MH et al. Misfolded proteins induce aggregation of the lectin Yos9. J Biol Chem 289:25670-7 (2014). WB . PubMed: 25086047
- Ma L et al. Serum anti-CCNY autoantibody is an independent prognosis indicator for postoperative patients with early-stage nonsmall-cell lung carcinoma. Dis Markers 35:317-25 (2013). WB . PubMed: 24167380
- Ma Z et al. Targeting human epidermal growth factor receptor signaling with the neuregulin's heparin-binding domain. J Biol Chem 284:32108-15 (2009). ICC/IF . PubMed: 19717564
- Bergman LM et al. Role of the unique N-terminal domain of CtBP2 in determining the subcellular localisation of CtBP family proteins. BMC Cell Biol 7:35 (2006). PubMed: 16999872