Anti-6X His tag® antibody [AD1.1.10] (Biotin) (ab15145)

Overview

  • Product name
    Anti-6X His tag® antibody [AD1.1.10] (Biotin)
    See all 6X His tag® primary antibodies
  • Description
    Mouse monoclonal [AD1.1.10] to 6X His tag® (Biotin)
  • Host species
    Mouse
  • Conjugation
    Biotin
  • Specificity
    The clone number has been updated from (3H2201) to (AD1.1.10) both clone numbers name the same antibody clone.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    His-His-His-His-His-His conjugated to KLH.

  • General notes
    HIS-TAG® is a trademark of EMD Biosciences, Inc.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    AD1.1.10
  • Myeloma
    NS1
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab15145 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000.
ELISA 1/500 - 1/1000.

Target

  • Relevance
    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • Cellular localization
    Depends upon the localization of the parent protein tagged with hexahistidine.
  • Alternative names
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

References

ab15145 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Question
Answer

The biotin binding of ab15145 has not been quantified, but it is typically in the range of 4-6 biotin molecules / antibody. The biotin is linked through lysine residues on the antibody surface.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up