Overview

  • Product name

    Anti-6X His tag® antibody [AD1.1.10] (DyLight® 488)
    See all 6X His tag® primary antibodies
  • Description

    Mouse monoclonal [AD1.1.10] to 6X His tag® (DyLight® 488)
  • Host species

    Mouse
  • Conjugation

    DyLight® 488. Ex: 493nm, Em: 518nm
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, IHC-Frmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Synthetic peptide of 6X His tag®.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, 0.8766% Sodium chloride, 81% Dibasic monohydrogen sodium phosphate, 19% Monobasic dihydrogen sodium phosphate
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    AD1.1.10
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab117512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/5000.
Flow Cyt Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 25285446

Target

  • Relevance

    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • Cellular localization

    Depends upon the localization of the parent protein tagged with hexahistidine.
  • Alternative names

    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

References

This product has been referenced in:

  • Imhof BA  et al. CCN1/CYR61-mediated meticulous patrolling by Ly6Clow monocytes fuels vascular inflammation. Proc Natl Acad Sci U S A 113:E4847-56 (2016). Read more (PubMed: 27482114) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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