Anti-6X His tag® antibody [AD1.1.10] (SureLight® Allophycocyanin) (ab133714)

Overview

  • Product name

    Anti-6X His tag® antibody [AD1.1.10] (SureLight® Allophycocyanin)
    See all 6X His tag® primary antibodies
  • Description

    Mouse monoclonal [AD1.1.10] to 6X His tag® (SureLight® Allophycocyanin)
  • Host species

    Mouse
  • Conjugation

    SureLight® Allophycocyanin. Ex: 652nm, Em: 657nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Polyhistidine tagged peptide

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Store In the Dark.
  • Storage buffer

    pH: 8.20
    Constituents: 0.12% Tris, 1.7% Sucrose, 0.88% Sodium chloride, 0.1% BSA
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    AD1.1.10
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab133714 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab73711 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Relevance

    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • Cellular localization

    Depends upon the localization of the parent protein tagged with hexahistidine.
  • Alternative names

    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

References

This product has been referenced in:

  • Andersen TK  et al. Enhanced germinal center reaction by targeting vaccine antigen to major histocompatibility complex class II molecules. NPJ Vaccines 4:9 (2019). Read more (PubMed: 30775000) »
See 1 Publication for this product

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