Product nameAnti-6X His tag® antibody (Agarose)
See all 6X His tag® primary antibodies
DescriptionGoat polyclonal to 6X His tag® (Agarose)
Tested applicationsSuitable for: IPmore details
Unsuitable for: ChIP
General notesHIS-TAG® is a trademark of EMD Biosciences, Inc.
Affinity purified antibodies were coupled to agarose beads using a cyanogen bromide method.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 0.0268% PBS, 0.58% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab1231 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.
Use 20-40 µl of gel slurry per 0.1-1 mg of protein lysate or extract.
RelevanceThe H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Cellular localizationDepends upon the localization of the parent protein tagged with hexahistidine.
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
ab1231 has not yet been referenced specifically in any publications.