Western blot abreview for Anti-6X His tag® antibody - ChIP Grade

Excellent
Abreviews
Application
Western blot
Sample
Human Purified protein (His-tagged purified protein (histone H2B))
Loading amount
0.5 µg
Specification
His-tagged purified protein (histone H2B)
Gel Running Conditions
Reduced Denaturing (15%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C

Other product details

Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: BSA 3% in PBST

Secondary antibody

Name
Non-Abcam antibody was used: Jackson Goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/15000

Detection

Detection method
ECL
Exposure
20 second(s)
Bands
Specific: 17 kDa
Positive control
Different amounts of purified His-tagged H2B protein were loaded on a 15% gel (amounts: 50ng, 500ng, 5000ng).

Dr. Efrat Shema

Verified customer

Submitted Aug 11 2011

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