Western blot abreview for Anti-6X His tag® antibody - ChIP Grade

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293 cells)
Loading amount
20 µg
Specification
HEK293 cells
Gel Running Conditions
Non-reduced Denaturing (gel 12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Other product details

Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 24°C · Diluent: 5 % milk

Secondary antibody

Name
Non-Abcam antibody was used: anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/4000

Detection

Detection method
ECL+
Exposure
15 second(s)
Bands
Specific: 26 kDa
Negative control
Hisx6 EV

Additional data

Additional Notes
Cells were transfected with flag-tag protein + Hisx6-ubq or hisx6 EV (-ve). His pulldown dynabeads we're used to pull down Hisx6ubq with flag-binding protein. Membranes we're blotted with abcam hisx6 ab9108 to detect mono-ubq of target protein at 26KDa.

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Submitted Nov 11 2011

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