Cells were transfected with flag-tag protein + Hisx6-ubq or hisx6 EV (-ve). His pulldown dynabeads we're used to pull down Hisx6ubq with flag-binding protein. Membranes we're blotted with abcam hisx6 ab9108 to detect mono-ubq of target protein at 26KDa.
Abcam user community
Submitted Nov 11 2011
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