Western blot abreview for Anti-6X His tag® antibody - ChIP Grade

Good
Abreviews
Application
Western blot
Sample
Mouse Recombinant protein (HEK Cell supernatant)
Loading amount
40 µg
Specification
HEK Cell supernatant
Gel Running Conditions
Non-reduced Denaturing (15)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C

Other product details

Dilution
1/4000
Incubation time
10 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST

Secondary antibody

Name
Non-Abcam antibody was used: HRP coupled anti Rabbit
Host species: Donkey
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL+
Exposure
1 minute(s) and 10 second(s)
Bands
Specific: 25 kDa

Additional data

Additional Notes
1 Input
2 Flow Through
3 First Wash
4 Second Wash
5 Elution

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Verified customer

Submitted Sep 13 2012

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