Immunoprecipitation abreview for Anti-6X His tag® antibody - ChIP Grade

Excellent
Abreviews
Application
Immunoprecipitation
Sample
Human Cell lysate - other (HEK Cell memrane lysate incubated with supernatant)
Total protein in input
100000 cells
Specification
HEK Cell memrane lysate incubated with supernatant
Immuno-precipitation step
Protein G

Other product details

Dilution
1/1000
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 4°C

Western Blot antibody

Name
Non-Abcam antibody was used: Myc tag antibody
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000

Additional data

Additional Notes
lane 1: supernatant (positive control)
lane 2: IP. Lower bands represents input and the upper band represents co-immunoprecipitated partner.
Lane 3: IP without partner

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Submitted Feb 01 2013

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