Western blot abreview for Anti-6X His tag® antibody - ChIP Grade

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Abreviews
Application
Western blot
Sample
Escherichia coli Cell lysate - whole cell (membrane pellet homogenised)
Loading amount
10 µg
Specification
membrane pellet homogenised
Gel Running Conditions
Reduced Denaturing (10-20% Tricine)
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: TBST

Secondary antibody

Name
Non-Abcam antibody was used: goat anti rabbit conjugate alexa fluor
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Dilution
1/10000

Detection

Detection method
lycor
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 65 kDa Non-specific: 100, 34, 25 aprox kDa

Additional data

Additional Notes
Apart from some unspecific binding a good antibody which detected a his tagged fusion protein which was expressed in e.coli.

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Submitted Mar 12 2013

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