Western blot abreview for Anti-6X His tag® antibody - ChIP Grade

Good
Abreviews
Application
Western blot
Sample
African Green Monkey Recombinant protein (COS 7 cells)
Loading amount
10 µg
Specification
COS 7 cells
Treatment
Homogenised
Gel Running Conditions
Reduced Denaturing (12% tris glycine)
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: TBS

Secondary antibody

Name
Non-Abcam antibody was used: goat anti rabbit conjugate 647
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Dilution
1/10000

Detection

Detection method
LYCOR
Exposure
5 minute(s) and 0 second(s)

Additional data

Additional Notes
A good antibody which detected a his tagged protein expressed in COS 7 cells

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Verified customer

Submitted Mar 27 2013

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