Western blot abreview for Anti-6X His tag® antibody - ChIP Grade

Excellent
Abreviews
Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Human Cell lysate - whole cell (HeLa)
Specification
HeLa
Treatment
transfected with His-tagged gene
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C

Other product details

Incubation time
24 hour(s) and 0 minute(s) · Temperature: 23°C
Dilution
1/4000

Secondary antibody

Name
Non-Abcam antibody was used: anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL
Bands
Specific: 35 kDa
Exposure
30 second(s)
Positive control
Whole cell lysate of HeLa transfected with His-tagged gene

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Submitted Mar 29 2014

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