Immunoprecipitation abreview for Anti-6X His tag® antibody - ChIP Grade

Excellent
Abreviews
Application
Immunoprecipitation
Immuno-precipitation step
Protein G
Sample
Human Cell lysate - whole cell (HeLa)
Specification
HeLa
Treatment
100ug/ml FAC or 100uM DFO for 8hrs
Total protein in input
5 µg

Other product details

Incubation time
24 hour(s) and 0 minute(s) · Temperature: 23°C
Dilution
1/200

Western Blot antibody

Name
Non-Abcam antibody was used: Goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Abcam user community

Verified customer

Submitted Apr 18 2014

Sign up