ELISA abreview for Anti-6X His tag® antibody [EPR20547] - ChIP Grade

Poor
Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
ELISA
Sample
Human Recombinant protein (growth factor)
Specification
growth factor
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Type
Direct

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: blocking buffer
Dilution
1/1000

Secondary antibody

Dilution
1/30000
Name
Non-Abcam antibody was used: anti-Rabbit IgG (whole molecule) - Peroxidase, ant
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Additional data

Additional Notes
The antibody was used againts 0.5 ug of protein coated on ELISA plate. Tested protein always obtained His-tag antibody was not tested against protein without His-tag.
Abcam response
We would like to thank the reviewer for submitting this AbReview. As the product has not yet been tested in this application, we were unsure what the result might be and we appreciate the time and effort that the reviewer has put in to testing this product in this application. We encourage all our customers to contact us should they wish to test any of our products in untested species or applications, as these may qualify for our AbTrial testing program.

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Verified customer

Submitted Mar 15 2018

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