Product nameAnti-6X His tag® antibody (FITC)
See all 6X His tag® primary antibodies
DescriptionRabbit polyclonal to 6X His tag® (FITC)
ConjugationFITC. Ex: 493nm, Em: 528nm
Tested applicationsSuitable for: ELISA, WB, Flow Cytmore details
Synthetic peptide: HHHHHH conjugated to KLH.
HIS-TAG® is a trademark of EMD Biosciences, Inc.
Molar F/P ratio is 3.7:1.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.2
Constituents: PBS, 0.2% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to fluorescein isothiocyanate.
Our Abpromise guarantee covers the use of ab1206 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. PubMed: 21183632|
|Flow Cyt||Use at an assay dependent concentration.
ab37406 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
RelevanceThe H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Cellular localizationDepends upon the localization of the parent protein tagged with hexahistidine.
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
Muc1-Bi-1 and Muc1-Bi-2 can recognize Muc1 positive tumor cells.
a). Flow cytometry analysis of Muc1-Bi-1 and Muc1-Bi-2 on Muc1-negative cell lines HePG2 and CHO and Muc1-positive cell lines, HT29. Red line indicates cells with no staining; green line indicates cells with only anti-His-FITC staining (ab1206); blue line indicates cells with positive control anti-Muc1 antibody; purple line indicates cells with Muc1-Bi-1 protein; orange line indicates cells with Muc1-Bi-2 protein.
ab1206 staining 6X His tag® in human endometrial carcinoma cells by Flow Cytometry.
Cells were prepared by short trypsinisation in PBS/1% FBS/0.2% sodium azide and fixed with formaldehyde. The gate included all single cells. The sample was incubated with a 6X His tag® primary antibody for 1 hour at 4°C. ab1206 was used as the secondary. On the image, the red line represents cells stained with ab1206 alone, and the blue line is the tested sample carrying a 6XHis tag®. The small peak to the right represents cells that bound the tested sample.
This product has been referenced in:
- Li Y et al. Single domain based bispecific antibody, Muc1-Bi-1, and its humanized form, Muc1-Bi-2, induce potent cancer cell killing in muc1 positive tumor cells. PLoS One 13:e0191024 (2018). Read more (PubMed: 29357376) »
- Woller SA et al. Inhibition of Neuroinflammation by AIBP: Spinal Effects upon Facilitated Pain States. Cell Rep 23:2667-2677 (2018). Flow Cyt ; Mouse . Read more (PubMed: 29847797) »