Overview

  • Product name
    Anti-6X His tag® antibody (FITC)
    See all 6X His tag® primary antibodies
  • Description
    Rabbit polyclonal to 6X His tag® (FITC)
  • Host species
    Rabbit
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Tested applications
    Suitable for: ELISA, WB, Flow Cytmore details
  • Immunogen

    Synthetic peptide: HHHHHH conjugated to KLH.

  • General notes

    HIS-TAG® is a trademark of EMD Biosciences, Inc.

    Molar F/P ratio is 3.7:1.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.2
    Constituents: PBS, 0.2% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to fluorescein isothiocyanate.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab1206 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. PubMed: 21183632
Flow Cyt Use at an assay dependent concentration.

ab37406 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Relevance
    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • Cellular localization
    Depends upon the localization of the parent protein tagged with hexahistidine.
  • Alternative names
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

Images

  • Muc1-Bi-1 and Muc1-Bi-2 can recognize Muc1 positive tumor cells.

    a). Flow cytometry analysis of Muc1-Bi-1 and Muc1-Bi-2 on Muc1-negative cell lines HePG2 and CHO and Muc1-positive cell lines, HT29. Red line indicates cells with no staining; green line indicates cells with only anti-His-FITC staining (ab1206); blue line indicates cells with positive control anti-Muc1 antibody; purple line indicates cells with Muc1-Bi-1 protein; orange line indicates cells with Muc1-Bi-2 protein.

  • ab1206 staining 6X His tag® in human endometrial carcinoma cells by Flow Cytometry.

    Cells were prepared by short trypsinisation in PBS/1% FBS/0.2% sodium azide and fixed with formaldehyde. The gate included all single cells. The sample was incubated with  a 6X His tag® primary antibody for 1 hour at 4°C. ab1206 was used as the secondary. On the image, the red line represents cells stained with ab1206 alone, and the blue line is the tested sample carrying a 6XHis tag®.  The small peak to the right represents cells that bound the tested sample.

    See Abreview

References

This product has been referenced in:
  • Li Y  et al. Single domain based bispecific antibody, Muc1-Bi-1, and its humanized form, Muc1-Bi-2, induce potent cancer cell killing in muc1 positive tumor cells. PLoS One 13:e0191024 (2018). Read more (PubMed: 29357376) »
  • Woller SA  et al. Inhibition of Neuroinflammation by AIBP: Spinal Effects upon Facilitated Pain States. Cell Rep 23:2667-2677 (2018). Flow Cyt ; Mouse . Read more (PubMed: 29847797) »
See all 13 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Spodoptera frugiperda (SF9 cells) Cell (Baculovirus infected-cells. Baculovirus having a f)
Permeabilization
Yes - Saponin
Gating Strategy
Single cells (FS-A vs FS-H) then SS-A vs FS-A
Specification
Baculovirus infected-cells. Baculovirus having a f
Preparation
Cell harvesting/tissue preparation method: Cell culture gently pipetting up and down
Sample buffer: Pelleted in their own culture medium
Fixation
Formaldehyde

Dr. Waldo Medina

Verified customer

Submitted May 02 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (Detection of a HIS tagged protein bound to cells)
Specification
Detection of a HIS tagged protein bound to cells
Preparation
Cell harvesting/tissue preparation method: Trypsinisation and pre-incubation with HIS-tagged protein
Sample buffer: Serum free DMEM
Fixation
Nonne
Permeabilization
No
Gating Strategy
Live cell population

Abcam user community

Verified customer

Submitted Jul 02 2012

Answer

I've assembled a list of the items that you would need forDengue Virus NS1 glycoprotein detection. Capture antibody ab41646,Anti-Dengue Virus NS1 glycoprotein antibody [DN3] Mouse monoclonal: https://www.abcam.com/Dengue-Virus-NS1-glycoprotein-antibody-DN3-ab41616.html Dengue Virus NS1 glycoproteinProtein, ab64456. This is a His tagged protein which allows for a greater selection of detection antibodies and thereby give you options for detection substrates: https://www.abcam.com/Dengue-Virus-NS1-glycoprotein-protein-His-tag-ab64456.html Detection antibody. These are all rabbit polyclonal with differing labels that you might use with different detection systems: FITC labeled:https://www.abcam.com/6X-His-tag-antibody-FITC-ab1206.html HRP labeled:https://www.abcam.com/6X-His-tag-antibody-HRP-ab1187.html, you would need to purchase an HRP chromogen substrate for detection using this. Biotin labeled:https://www.abcam.com/6X-His-tag-antibody-Biotin-ab27025.html,you would need to purchase a streptavidin conjugated chromogen substrate system for detection using this. You may want to use ab41646, the 'capture' antibody for 'detection' instead. To do that you will need to conjugate a tag to the antibody. We have a number of products available for that. Here is the link to all of our EasyLink Conjugation kits: https://www.abcam.com/index.html?pageconfig=resource&rid=13148&source=pagetrap&viapagetrap=easylink A link to the FAQs for EasyLink, please review these as they contain very helpful information:https://www.abcam.com/index.html?pageconfig=resource&rid=13156 We offer 3 sizes for FITC conjugation, I've linked those below: https://www.abcam.com/EasyLink-FITC-Conjugation-Kit-3-x-10%C2%B5g-FITC-ab102885.html https://www.abcam.com/EasyLink-FITC-Conjugation-Kit-3-x-100%C2%B5g-FITC-ab102884.html https://www.abcam.com/EasyLink-FITC-Conjugation-Kit-3-x-100%C2%B5g-FITC-ab102884.html

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (A431)
Specification
A431
Preparation
Cell harvesting/tissue preparation method: trypsin/EDTA
Sample buffer: PBS + 1 g/L BSA
Fixation
none
Permeabilization
No
Gating Strategy
no gating

Dr. Benjamin Hackel

Verified customer

Submitted Sep 14 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (endometrial carcinoma)
Specification
endometrial carcinoma
Preparation
Cell harvesting/tissue preparation method: short trypsinisation
Sample buffer: PBS+1%FBS+0.02%NaN3
Fixation
Formaldehyde
Permeabilization
No
Gating Strategy
gate included all single cells

Abcam user community

Verified customer

Submitted Oct 07 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Pichia pastoris Recombinant protein (yeast cell)
Specification
yeast cell
Blocking step
BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Type
Indirect

Abcam user community

Verified customer

Submitted May 14 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Saccharomyces cerevisiae Cell (yeast cells expressing a his tag on surface)
Specification
yeast cells expressing a his tag on surface
Permeabilization
No
Gating Strategy
gate included all yeast, both expressing and non-expressing

Abcam user community

Verified customer

Submitted Jun 04 2008

Question
Answer

The molecular weight of Rabbit IgG is ~150,000.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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