Overview

  • Product name
    Anti-6X His tag® antibody (FITC)
    See all 6X His tag® primary antibodies
  • Description
    Rabbit polyclonal to 6X His tag® (FITC)
  • Host species
    Rabbit
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Tested applications
    Suitable for: ELISA, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Synthetic peptide: HHHHHH conjugated to KLH.

  • General notes

    HIS-TAG® is a trademark of EMD Biosciences, Inc.

    Molar F/P ratio is 3.7:1.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.2
    Constituents: PBS, 0.2% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to fluorescein isothiocyanate.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab1206 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. PubMed: 21183632
Flow Cyt Use at an assay dependent concentration.

ab37406 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Relevance
    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • Cellular localization
    Depends upon the localization of the parent protein tagged with hexahistidine.
  • Alternative names
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

Images

  • Muc1-Bi-1 and Muc1-Bi-2 can recognize Muc1 positive tumor cells.

    a). Flow cytometry analysis of Muc1-Bi-1 and Muc1-Bi-2 on Muc1-negative cell lines HePG2 and CHO and Muc1-positive cell lines, HT29. Red line indicates cells with no staining; green line indicates cells with only anti-His-FITC staining (ab1206); blue line indicates cells with positive control anti-Muc1 antibody; purple line indicates cells with Muc1-Bi-1 protein; orange line indicates cells with Muc1-Bi-2 protein.

  • ab1206 staining 6X His tag® in human endometrial carcinoma cells by Flow Cytometry.

    Cells were prepared by short trypsinisation in PBS/1% FBS/0.2% sodium azide and fixed with formaldehyde. The gate included all single cells. The sample was incubated with  a 6X His tag® primary antibody for 1 hour at 4°C. ab1206 was used as the secondary. On the image, the red line represents cells stained with ab1206 alone, and the blue line is the tested sample carrying a 6XHis tag®.  The small peak to the right represents cells that bound the tested sample.

    See Abreview

References

This product has been referenced in:
  • Li Y  et al. Single domain based bispecific antibody, Muc1-Bi-1, and its humanized form, Muc1-Bi-2, induce potent cancer cell killing in muc1 positive tumor cells. PLoS One 13:e0191024 (2018). Read more (PubMed: 29357376) »
  • Woller SA  et al. Inhibition of Neuroinflammation by AIBP: Spinal Effects upon Facilitated Pain States. Cell Rep 23:2667-2677 (2018). Flow Cyt ; Mouse . Read more (PubMed: 29847797) »
See all 13 Publications for this product

Customer reviews and Q&As

1-9 of 9 Abreviews or Q&A

Application
Western blot
Sample
Fall armyworm Recombinant protein (SF21 insect cells)
Gel Running Conditions
Reduced Denaturing (10% SDS PAGE)
Loading amount
10 µg
Specification
SF21 insect cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 20°C

Ms. Samira Acajjaoui

Verified customer

Submitted Jan 28 2019

Application
Flow Cytometry
Sample
Spodoptera frugiperda (SF9 cells) Cell (Baculovirus infected-cells. Baculovirus having a f)
Permeabilization
Yes - Saponin
Gating Strategy
Single cells (FS-A vs FS-H) then SS-A vs FS-A
Specification
Baculovirus infected-cells. Baculovirus having a f
Preparation
Cell harvesting/tissue preparation method: Cell culture gently pipetting up and down
Sample buffer: Pelleted in their own culture medium
Fixation
Formaldehyde

Dr. Waldo Medina

Verified customer

Submitted May 02 2016

Application
Flow Cytometry
Sample
Human Cell (Detection of a HIS tagged protein bound to cells)
Specification
Detection of a HIS tagged protein bound to cells
Preparation
Cell harvesting/tissue preparation method: Trypsinisation and pre-incubation with HIS-tagged protein
Sample buffer: Serum free DMEM
Fixation
Nonne
Permeabilization
No
Gating Strategy
Live cell population

Abcam user community

Verified customer

Submitted Jul 02 2012

Answer

I've assembled a list of the items that you would need forDengue Virus NS1 glycoprotein detection. Capture antibody ab41646,Anti-Dengue Virus NS1 glycoprotein antibody [DN3] Mouse monoclonal: https://www.abcam.com/Dengue-Virus-NS1-glycoprotein-antibody-DN3-ab41616.html Dengue Virus NS1 glycoproteinProtein, ab64456. This is a His tagged protein which allows for a greater selection of detection antibodies and thereby give you options for detection substrates: https://www.abcam.com/Dengue-Virus-NS1-glycoprotein-protein-His-tag-ab64456.html Detection antibody. These are all rabbit polyclonal with differing labels that you might use with different detection systems: FITC labeled:https://www.abcam.com/6X-His-tag-antibody-FITC-ab1206.html HRP labeled:https://www.abcam.com/6X-His-tag-antibody-HRP-ab1187.html, you would need to purchase an HRP chromogen substrate for detection using this. Biotin labeled:https://www.abcam.com/6X-His-tag-antibody-Biotin-ab27025.html,you would need to purchase a streptavidin conjugated chromogen substrate system for detection using this. You may want to use ab41646, the 'capture' antibody for 'detection' instead. To do that you will need to conjugate a tag to the antibody. We have a number of products available for that. Here is the link to all of our EasyLink Conjugation kits: https://www.abcam.com/index.html?pageconfig=resource&rid=13148&source=pagetrap&viapagetrap=easylink A link to the FAQs for EasyLink, please review these as they contain very helpful information:https://www.abcam.com/index.html?pageconfig=resource&rid=13156 We offer 3 sizes for FITC conjugation, I've linked those below: https://www.abcam.com/EasyLink-FITC-Conjugation-Kit-3-x-10%C2%B5g-FITC-ab102885.html https://www.abcam.com/EasyLink-FITC-Conjugation-Kit-3-x-100%C2%B5g-FITC-ab102884.html https://www.abcam.com/EasyLink-FITC-Conjugation-Kit-3-x-100%C2%B5g-FITC-ab102884.html

Read More
Application
Flow Cytometry
Sample
Human Cell (A431)
Specification
A431
Preparation
Cell harvesting/tissue preparation method: trypsin/EDTA
Sample buffer: PBS + 1 g/L BSA
Fixation
none
Permeabilization
No
Gating Strategy
no gating

Dr. Benjamin Hackel

Verified customer

Submitted Sep 14 2010

Application
Flow Cytometry
Sample
Human Cell (endometrial carcinoma)
Specification
endometrial carcinoma
Preparation
Cell harvesting/tissue preparation method: short trypsinisation
Sample buffer: PBS+1%FBS+0.02%NaN3
Fixation
Formaldehyde
Permeabilization
No
Gating Strategy
gate included all single cells

Abcam user community

Verified customer

Submitted Oct 07 2009

Application
ELISA
Sample
Pichia pastoris Recombinant protein (yeast cell)
Specification
yeast cell
Blocking step
BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Type
Indirect

Abcam user community

Verified customer

Submitted May 14 2009

Application
Flow Cytometry
Sample
Saccharomyces cerevisiae Cell (yeast cells expressing a his tag on surface)
Specification
yeast cells expressing a his tag on surface
Permeabilization
No
Gating Strategy
gate included all yeast, both expressing and non-expressing

Abcam user community

Verified customer

Submitted Jun 04 2008

Question
Answer

The molecular weight of Rabbit IgG is ~150,000.

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