Key features and details
- Chicken polyclonal to 6X His tag® (FITC)
- Suitable for: WB, ELISA
- Reacts with: Species independent
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgY
Product nameAnti-6X His tag® antibody (FITC)
See all 6X His tag® primary antibodies
DescriptionChicken polyclonal to 6X His tag® (FITC)
ConjugationFITC. Ex: 493nm, Em: 528nm
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Species independent
Synthetic peptide: HHHHHH conjugated to KLH.
HIS-TAG® is a trademark of EMD Biosciences, Inc.
Molar F/P ratio is 3.6:1.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.00
Preservative: 0.1% Sodium azide
Constituent: 0.2% BSA
Concentration information loading...
PurityImmunogen affinity purified
Light chain typeunknown
Our Abpromise guarantee covers the use of ab3554 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.
Prior to conjugation, the antibodies were analyzed by Western Blot.
|ELISA||Use at an assay dependent concentration.
Prior to conjugation, the antibodies were analyzed by ELISA.
RelevanceThe H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Cellular localizationDepends upon the localization of the parent protein tagged with hexahistidine.
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
ab3554 has been referenced in 1 publication.
- Del Toro D et al. Structural Basis of Teneurin-Latrophilin Interaction in Repulsive Guidance of Migrating Neurons. Cell 180:323-339.e19 (2020). PubMed: 31928845