Key features and details
- Mouse monoclonal [HIS-1] to 6X His tag®
- Suitable for: Flow Cyt, WB, Dot blot, ELISA, ICC/IF
- Reacts with: Species independent
- Isotype: IgG2a
Product nameAnti-6X His tag® antibody [HIS-1]
See all 6X His tag® primary antibodies
DescriptionMouse monoclonal [HIS-1] to 6X His tag®
ab49939 preferentially reacts to recombinant proteins containing the 6X His tag® fused to amino terminus of fusion proteins.
Tested applicationsSuitable for: Flow Cyt, WB, Dot blot, ELISA, ICC/IFmore details
Species reactivityReacts with: Species independent
Recombinant full length protein corresponding to 6X His tag®.
This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot(s) is/are from ascites and is still in stock as of 25th October 2016 - GR224242. Lot numbers higher than GR224242 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
HIS-TAG® is a trademark of EMD Biosciences, Inc.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Concentration information loading...
Purification notesPurified from Tissue culture supernatant.
Our Abpromise guarantee covers the use of ab49936 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|
|Dot blot||Use at an assay dependent concentration.
Use at an assay dependent concentration.
|ELISA||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
RelevanceThe H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Cellular localizationDepends upon the localization of the parent protein tagged with hexahistidine.
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
ab49936 has been referenced in 1 publication.
- Allen ER et al. A Protective Monoclonal Antibody Targets a Site of Vulnerability on the Surface of Rift Valley Fever Virus. Cell Rep 25:3750-3758.e4 (2018). PubMed: 30590046