Product nameAnti-6X His tag® antibody [HIS-1] (Alkaline Phosphatase)
See all 6X His tag® primary antibodies
DescriptionMouse monoclonal [HIS-1] to 6X His tag® (Alkaline Phosphatase)
Tested applicationsSuitable for: WB, Dot blot, ELISAmore details
Species reactivityReacts with: Species independent
Recombinant poly(Histidine)-tagged fusion protein.
- Bacteria lysates expressing a recombinant polyHistidine tagged fusion protein and BCIP/NBT substrate.
HIS-TAG® is a trademark of EMD Biosciences, Inc.
Monoclonal antibodies reacting specifically with poly-histidine may be useful in various immunotechniques, to identify the expression of a polyhistidine fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a polyHistidinetagged fusion protein expressing vectors.
The antibody is prepared by conjugation of calf intestinal alkaline phosphatase to mouse monoclonal anti-polyHistidine purified from ascites fluid of the HIS-1 hybridoma
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 8.00
Preservative: 0.097% Sodium azide
Constituents: 0.06% Tris buffered saline, 0.0095% Magnesium chloride, 50% Glycerol, 1% BSA
Concentration information loading...
Primary antibody notesMonoclonal antibodies reacting specifically with poly-histidine may be useful in various immunotechniques, to identify the expression of a polyhistidine fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a polyHistidinetagged fusion protein expressing vectors.
Our Abpromise guarantee covers the use of ab49746 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 7 kDa.|
|Dot blot||Use at an assay dependent dilution.|
|ELISA||Use at an assay dependent dilution.|
RelevanceThe H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Cellular localizationDepends upon the localization of the parent protein tagged with hexahistidine.
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
This product has been referenced in:
- Henche AL et al. Dissection of key determinants of cleavage activity in signal peptidase III (SPaseIII) PibD. Extremophiles 18:905-13 (2014). Read more (PubMed: 25102813) »
- Hussack G et al. Neutralization of clostridium difficile toxin A with single-domain antibodies targeting the cell-receptor binding domain. J Biol Chem : (2011). WB . Read more (PubMed: 21216961) »