Biotin Anti-6X His tag® antibody [HIS.H8] (ab173828)
Key features and details
- Biotin Mouse monoclonal [HIS.H8] to 6X His tag®
- Suitable for: ELISA, IP, ICC/IF, WB
- Reacts with: Species independent
- Conjugation: Biotin
- Isotype: IgG2b
Related conjugates and formulations
Overview
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Product name
Biotin Anti-6X His tag® antibody [HIS.H8]
See all 6X His tag® primary antibodies -
Description
Biotin Mouse monoclonal [HIS.H8] to 6X His tag® -
Host species
Mouse -
Conjugation
Biotin -
Tested applications
Suitable for: ELISA, IP, ICC/IF, WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide corresponding to 6X His tag® aa 1-6.
Sequence:HHHHHH
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Positive control
- E. coli lysate containing a multi-epitope tagged protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
HIS.H8 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab173828 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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ICC/IF |
1/500 - 1/2000.
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WB |
1/500 - 1/1000. Predicted molecular weight: 0.8 kDa.
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Notes |
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ELISA
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
ICC/IF
1/500 - 1/2000. |
WB
1/500 - 1/1000. Predicted molecular weight: 0.8 kDa. |
Target
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Relevance
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. His-tags can be fused to the amino- or carboxy- termini of proteins in transfected or transformed cells. -
Cellular localization
Depends upon the localization of the parent protein tagged with hexahistidine. -
Alternative names
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
Images
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All lanes : Biotin Anti-6X His tag® antibody [HIS.H8] (ab173828) at 1/1000 dilution
Lane 1 : E. coli lysate containing a multi-epitope tagged protein at 0.5 µg
Lane 2 : E. coli lysate containing a multi-epitope tagged protein at 0.25 µg
Lane 3 : E. coli lysate containing a multi-epitope tagged protein at 0.125 µg
Lane 4 : E. coli lysate containing a multi-epitope tagged protein at 0.0599999986588955 µg
Lane 5 : E. coli lysate containing a multi-epitope tagged protein at 0.0299999993294477 µg
Lane 6 : E. coli lysate containing a multi-epitope tagged protein at 0.0149999996647239 µg
Secondary
All lanes : Streptavidin-HRP at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 0.8 kDaNote: Predicted MW is for the His tag alone.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab173828 has not yet been referenced specifically in any publications.